Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation (2uL of anti-JNK2) using 20ng of GST-JNK2 recombinant protein.)
Mouse JNK2 Monoclonal Antibody | anti-JNK2 antibody
Anti-JNK2
Reactivity
Immunoprecipitation of human JNK2Western blot of human JNK2.
JNK2 from other species may also be detectable.
Applications
Western Blot, Immunoprecipitation
Purity
Affinity purified using Protein G
Synonyms
JNK2; Monoclonal Antibody; Anti-JNK2; anti-JNK2 antibody
Host
Mouse
Reactivity
Immunoprecipitation of human JNK2
Western blot of human JNK2.
JNK2 from other species may also be detectable.
Western blot of human JNK2.
JNK2 from other species may also be detectable.
Clonality
Monoclonal
Isotype
Mouse, IgG2
Clone Number
2G12
Specificity
Recognizes only the JNK2 protein
Purity/Purification
Affinity purified using Protein G
Form/Format
10mM PBS pH7.2
Applicable Applications for anti-JNK2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 21994:22993 dilution
IP: 2ug
IP: 2ug
Immunogen
Human recombinant full length JNK2 protein
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation (2uL of anti-JNK2) using 20ng of GST-JNK2 recombinant protein.)
Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation (2uL of anti-JNK2) using 20ng of GST-JNK2 recombinant protein.)
Western Blot (WB)
(Sample Western Blot: Representative western blot (1:1000) using 20ng of GST-JNK1, GST-JNK2, and GST-JNK3 recombinant protein.)
Western Blot (WB)
(Sample Western Blot: Representative western blot (1:1000) using 20ng of GST-JNK1, GST-JNK2, and GST-JNK3 recombinant protein.)
Related Product Information for anti-JNK2 antibody
JNK2 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation and treatment of cells with TNFalpha. JNK2 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK2 has been shown to play an important role in disease processes. JNK2 plays a role in ventricular hypertrophy and is thought to be involved in hypertensive cardiac disease (2).
Product Categories/Family for anti-JNK2 antibody
References
1. Sluss, H K. et al: Signal transduction by tumor necrosis factor mediated by JNK protein kinases. Mol Cell Biol. 1994 Dec;14(12):8376-84.
2. Vogel, V. et al: Cardiac hypertrophy in the Prague-hypertensive rat is associated with enhanced JNK2 but not ERK tissue activity. Kidney Blood Press Res. 2001;24(1):52-6.
2. Vogel, V. et al: Cardiac hypertrophy in the Prague-hypertensive rat is associated with enhanced JNK2 but not ERK tissue activity. Kidney Blood Press Res. 2001;24(1):52-6.
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Product Notes
The JNK2 (Catalog #AAA516094) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-JNK2 reacts with Immunoprecipitation of human JNK2 Western blot of human JNK2. JNK2 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's JNK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 21994:22993 dilution IP: 2ug. Researchers should empirically determine the suitability of the JNK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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