Mouse JAZF1 Monoclonal Antibody | anti-JAZF1 antibody

JAZF1 (Transcription Corepressor) Mouse Monoclonal Antibody [Clone PCRP-JAZF1-1C2]

Cat. Num. AAA4381890

• Gene Names: JAZF1; TIP27; ZNF802
• Reactivity: Human; Predicted to react in Mouse.
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: JAZF1; Monoclonal Antibody; JAZF1 (Transcription Corepressor) Mouse Monoclonal Antibody [Clone PCRP-JAZF1-1C2]; JAZF1 (Transcription Corepressor); DKFZp761K2222; TIP27; ZNF802; anti-JAZF1 antibody

• Host: Mouse
• Reactivity: Human; Predicted to react in Mouse.
• Clonality: Monoclonal
• Isotype: Mouse/IgG2a
• Clone Number: PCRP-JAZF1-1C2
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-JAZF1 antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F)
• Application Notes: IP: 1-2ug per 100-500ug of total protein); FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml; WB: 1-2ug/ml; IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT; (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
• Immunogen: Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary)
• Cellular Localization: Nucleus
• Positive Control: HeLa or U87 cells. Ubiquitous tissue expression.
• Hu-chromosome Location: 7p15.2-p15.1
• Unigene: 368944
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2). Confirmation of Purity and Integrity of Antibody. )

Related Product Information for anti-JAZF1 antibody

JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 91822911
• NCBI GeneID: 221895
• NCBI Accession #: NP_778231.2
• NCBI GenBank Nucleotide #: NM_175061.3
• UniProt Accession #: Q86VZ6; Q8N3L7; A4D195
• Molecular Weight: 25,739 Da
• NCBI Official Full Name: juxtaposed with another zinc finger protein 1
• NCBI Official Synonym Full Names: JAZF zinc finger 1
• NCBI Official Symbol: JAZF1
• NCBI Official Synonym Symbols: TIP27; ZNF802
• NCBI Protein Information: juxtaposed with another zinc finger protein 1; TAK1-interacting protein 27; juxtaposed with another zinc finger gene 1; zinc finger protein 802
• UniProt Protein Name: Juxtaposed with another zinc finger protein 1
• Protein Family: Juxtaposed with another zinc finger protein
• UniProt Gene Name: JAZF1
• UniProt Synonym Gene Names: TIP27; ZNF802
• UniProt Entry Name: JAZF1_HUMAN

NCBI Description

This gene encodes a nuclear protein with three C2H2-type zinc fingers, and functions as a transcriptional repressor. Chromosomal aberrations involving this gene are associated with endometrial stromal tumors. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized [provided by RefSeq, Jul 2008]

Uniprot Description

JAZF1: Potential transcription factor. A chromosomal aberration involving JAZF1 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with SUZ12. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Oncoprotein; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 7p15.2-p15.1

Cellular Component: transcriptional repressor complex; nucleus

Molecular Function: nucleic acid binding; metal ion binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter