Mouse anti-Human ISG15/UCRP Monoclonal Antibody | anti-ISG15/UCRP antibody

ISG15/UCRP

Cat. Num. AAA200506

• Gene Names: ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: By protein-A affinity chromatography
• Synonyms: ISG15/UCRP; Monoclonal Antibody; Interferon-stimulated gene 15 kDa ubiquitin-like modifier; GIP2; uCRP; IFI15; ISG15; Interferon-stimulated gene; 15 kDa ubiquitin-like modifier; 15 kDa ubiquitin-like modifier IFI 15; Interferon alpha inducible protein; Interferon induced 15 kDaprotein; Interferon induced 17 kDa protein; Interferon induced 17 kDa/15 kDa protein; InterferonStimulated Gene 15; Interferon stimulated protein 15 kDa; ISG 15; ISG15 ubiquitin like modifier; ubiquitin cross reactive protein precursor; anti-ISG15/UCRP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 3E5
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 165

• Applicable Applications for anti-ISG15/UCRP antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Immunogen: Recombinant human ISG15 (1-157aa) purified from E. coli
• Conjugation: Unconjugated
• Product Note: This product was produced from tissue culture supernatant.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Immunocytochemistry/Immunofluorescence (ICC/IF)

(ICC/IF analysis of ISG15 in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Western Blot (WB)

(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: MCF7 cell lysate;;Lane 2.: PC3 cell lysate;;)

Related Product Information for anti-ISG15/UCRP antibody

ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play importantroles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety ofproteins when cells are treated with type I interferon or lipopolysaccharide.

Product Categories/Family for anti-ISG15/UCRP antibody

Epigenetics and Nuclear Signaling; Immunology

References

Jonathan D et al.,(1996) Proc Natl Acad Sci USA 93:211-215.;;Weiming Y et al.,(2001) EMBO J 20(3):362-371.;;Chen Z et al.,(2005) Proc Natl Acad Sci USA 102(29):10200-10205.;;

NCBI and Uniprot Product Information

• NCBI GI #: 4826774
• NCBI GeneID: 9636
• NCBI Accession #: NP_005092.1
• NCBI GenBank Nucleotide #: NP_005092.1
• UniProt Accession #: P05161
• NCBI Official Full Name: ubiquitin-like protein ISG15
• NCBI Official Synonym Full Names: ISG15 ubiquitin like modifier
• NCBI Official Symbol: ISG15
• NCBI Official Synonym Symbols: G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• NCBI Protein Information: ubiquitin-like protein ISG15
• UniProt Protein Name: Ubiquitin-like protein ISG15
• UniProt Gene Name: ISG15
• UniProt Synonym Gene Names: G1P2; UCRP; IP17; hUCRP
• UniProt Entry Name: ISG15_HUMAN

NCBI Description

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]

Uniprot Description

ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: extracellular region; cytosol

Molecular Function: protein tag; protein binding

Biological Process: modification-dependent protein catabolic process; ISG15-protein conjugation; viral reproduction; negative regulation of viral genome replication; regulation of interferon-gamma production; cytokine and chemokine mediated signaling pathway; positive regulation of erythrocyte differentiation; defense response to bacterium; innate immune response; negative regulation of protein ubiquitination; defense response to virus; negative regulation of interferon type I production

Disease: Immunodeficiency 38