Human Ubiquitin-like protein ISG15 ELISA Kit | ISG15 elisa kit

Human Ubiquitin-like protein ISG15 ELISA Kit

Cat. Num. AAA764936

• Gene Names: ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• Reactivity: Human
• Synonyms: Ubiquitin-like protein ISG15; Human Ubiquitin-like protein ISG15 ELISA Kit; ISG15 (Interferon Stimulated Gene 15)/UCRP/G1P2/IFI15/IP17/G1P2interferon-induced 17-kDa/15-kDa protein/interferon; alpha-inducible protein (clone IFI-15K)/Interferon-induced 15 kDa protein/Interferon-induced 17 kDa protein/interferon-stimulated protein; 15 kDa/ISG15 ubiquitin-like modifier/Ubiquitin cross-reactive protein/ubiquitin-like protein ISG15/UCRPIP17; ISG15 elisa kit

• Reactivity: Human
• Sequence Length: 165

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ISG15 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ISG15 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ISG15 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ISG15 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ISG15 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4826774
• NCBI GeneID: 9636
• NCBI Accession #: NP_005092.1
• NCBI GenBank Nucleotide #: NM_005101.3
• Molecular Weight: 17,888 Da
• NCBI Official Full Name: ubiquitin-like protein ISG15
• NCBI Official Synonym Full Names: ISG15 ubiquitin-like modifier
• NCBI Official Symbol: ISG15
• NCBI Official Synonym Symbols: G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• NCBI Protein Information: ubiquitin-like protein ISG15
• UniProt Protein Name: Ubiquitin-like protein ISG15
• Protein Family: Ubiquitin-like protein
• UniProt Gene Name: ISG15
• UniProt Synonym Gene Names: G1P2; UCRP; IP17; hUCRP
• UniProt Entry Name: ISG15_HUMAN

NCBI Description

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]

Uniprot Description

ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytosol; extracellular region; nucleoplasm

Molecular Function: protein binding; protein tag

Biological Process: bypass DNA synthesis; cytokine and chemokine mediated signaling pathway; defense response to bacterium; defense response to virus; DNA repair; innate immune response; ISG15-protein conjugation; modification-dependent protein catabolic process; negative regulation of interferon type I production; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of erythrocyte differentiation; regulation of interferon-gamma production; viral reproduction

Disease: Immunodeficiency 38