IRAK4 / IRAK-4 recombinant protein

Recombinant Human IRAK4 / IRAK-4 Protein (His tag)

Cat. Num. AAA2545087

• Gene Names: IRAK4; IPD1; REN64; IRAK-4; NY-REN-64
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: IRAK4 / IRAK-4; Recombinant Human IRAK4 / IRAK-4 Protein (His tag); IPD1; NY-REN-64; REN64; IRAK4 / IRAK-4 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, pH 7.4
• Sequence Length: 460

• Application Notes: The recombinant human IRAK4 consists of 478 amino acids and predicts a molecular mass of 53.8 kDa. It migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IRAK4 / IRAK-4 recombinant protein

Background: Interleukin-1 receptor-associated kinase 4, also known as Renal carcinoma antigen NY-REN-64, IRAK-4 and IRAK4, is a member of the protein kinase superfamily, TKL Ser/Thr protein kinase family and Pelle subfamily. IRAK4 contains one death domain and one protein kinase domain. IRAK4 is required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. It also phosphorylates IRAK1. A member of the IL-1 receptor (IL-1R)-associated kinase (IRAK) family, IRAK4, has been shown to play an essential role in Toll-like receptor (TLR)-mediated signaling. IL-1-mediated IRAK4 kinase activity in T cells is essential for induction of IL-23R expression, Th17 differentiation, and autoimmune disease. Pharmacological blocking of IRAK4 kinase activity will retain some levels of host defence, while reducing the levels and duration of inflammatory responses, which should provide beneficial therapies for sepsis and chronic inflammatory diseases. Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) which is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotype s or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD. Defects in IRAK4 are also the cause of IRAK4 deficiency which causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.

Description: A DNA sequence encoding the full length of human IRAK4 (Q9NWZ3-1) (Met 1-Ser 460) was expressed with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 166795295
• NCBI GeneID: 51135
• NCBI Accession #: NP_001107654.1
• NCBI GenBank Nucleotide #: NM_001114182.2
• UniProt Accession #: Q9NWZ3; Q69FE1; Q8TDF7; Q9Y589
• Molecular Weight: 37,674 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 4 isoform a
• NCBI Official Synonym Full Names: interleukin 1 receptor associated kinase 4
• NCBI Official Symbol: IRAK4
• NCBI Official Synonym Symbols: IPD1; REN64; IRAK-4; NY-REN-64
• NCBI Protein Information: interleukin-1 receptor-associated kinase 4
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 4
• UniProt Gene Name: IRAK4
• UniProt Synonym Gene Names: IRAK-4

NCBI Description

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: EC 2.7.11.1; IRAK family; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: cytoplasm; cytosol; endosome membrane; nucleus; plasma membrane

Molecular Function: protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: innate immune response; MyD88-dependent toll-like receptor signaling pathway; neutrophil mediated immunity; positive regulation of I-kappaB kinase/NF-kappaB cascade; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency