Mouse anti-Human Interleukin-6 Monoclonal Antibody | anti-IL-6 antibody

Monoclonal Mouse Anti Human Interleukin-6

Cat. Num. AAA550031

• Reactivity: Human
• Purity: The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
• Synonyms: Interleukin-6; Monoclonal Antibody; Monoclonal Mouse Anti Human Interleukin-6; il-6; il6; interleukin-6; B-cell stimulatory factor; bsf; monoclonal; mab; anti-IL-6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SB9H6
• Purity/Purification: The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
• Form/Format: Prepared from sterile filtered solution at a concentration of 1mg/ml in PBS with 5% trehalose.
• Sequence Length: 212

• Application Notes: Direct ELISA: This antibody can be used at 0.5-1ug/mLwith the appropriate secondary reagents to detect human Interleukin-6. The detection limit for human IL-6 is approximately 2ng/well.; Western blot: This monoclonal antibody has been tested in WB analysis of human IL-6. A suitable range of concentrations of this antibody for WB detection is 0.5-2 ug/ml.
• Host Note: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human IL-6.
• Preparation and Storage: The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.; Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for anti-IL-6 antibody

IL6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL6 after stimulation. IL6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT- 1. All known members of the IL6 cytokine family induce hepatic expression of acute phase proteins. The IL6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T- cells. The IL6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.

Product Categories/Family for anti-IL-6 antibody

Monoclonal

References

• IL-6 protects against hyperoxia-induced mitochondrial damage via bcl-2–induced bak interactions with mitofusions .Am. J. Respir. Cell Mol. Biol., Oct 2009; 41: 385 - 396.
• long-term safety and efficacy of tocilizumab, an anti-IL-6 receptor monoclonal antibody, in monotherapy, in patients with rheumatoid arthritis (the stream study) : evidence of safety and efficacy in a 5-year extension study .Ann Rheum Dis, Oct 2009; 68: 1580 - 1584.
• the second bovine β-galactoside-2,6-sialyltransferase (st6gal ii) : genomic organization and stimulation of its in vitro expression by IL-6 in bovine mammary epithelial cells .Glycobiology, Oct 2009; 19: 1082 - 1093.
• IL-6 -174g/c promoter polymorphism in acute appendicitis .Lab Med, Oct 2009; 40: 600 - 603.
• cell surface-bound il-1 is an upstream regulator of the senescence-associated IL-6/il-8 cytokine network .PNAS, Sep 2009; 10.1073/pnas.0905299106.