Rat anti-Mouse Interleukin 17E Monoclonal Antibody | anti-IL25 antibody
Interleukin 17E (IL-17E, IL25, IL-25, Precursor, interleukin 25, Interleukin-25)
Gene Names
IL25; IL17E
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Interleukin 17E; Monoclonal Antibody; Interleukin 17E (IL-17E; IL25; IL-25; Precursor; interleukin 25; Interleukin-25); Anti -Interleukin 17E (IL-17E; anti-IL25 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8H118
Specificity
Recognizes mouse IL-17E. In sandwich ELISAs no crossreactivity or interference was observed with rhIL-17E, rmIL-17, rmIL-17B, C, D, or F.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile 40-50% glycerol, PBS.
Applicable Applications for anti-IL25 antibody
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA and Flow Cytometry.
Dilution: ELISA (Capture): 2-8ug/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard.
Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome.
Dilution: ELISA (Capture): 2-8ug/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard.
Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome.
Immunogen
E. coli-derived rmIL-17E
Hybridoma
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse IL-17E (rmIL-17E; aa 17-169; Accession # NP_542767).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-IL25 antibody
Interleukin 17E (IL-17E) is a disulfide-linked dimer that belongs to the IL-17 family of pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions.
Product Categories/Family for anti-IL25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,330 Da
NCBI Official Full Name
interleukin-25 isoform 2
NCBI Official Synonym Full Names
interleukin 25
NCBI Official Symbol
IL25
NCBI Official Synonym Symbols
IL17E
NCBI Protein Information
interleukin-25; interleukin-17E
UniProt Protein Name
Interleukin-25
Protein Family
UniProt Gene Name
IL25
UniProt Synonym Gene Names
IL17E; IL-25; IL-17E
UniProt Entry Name
IL25_HUMAN
NCBI Description
The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Uniprot Description
IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Cytokine
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: extracellular space
Molecular Function: cytokine activity; interleukin-17E receptor binding
Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter
Research Articles on IL25
Similar Products
Product Notes
The IL25 il25 (Catalog #AAA601150) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Interleukin 17E (IL-17E, IL25, IL-25, Precursor, interleukin 25, Interleukin-25) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 17E can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Flow Cytometry (FC/FACS). Suitable for use in ELISA and Flow Cytometry. Dilution: ELISA (Capture): 2-8ug/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard. Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome. Researchers should empirically determine the suitability of the IL25 il25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 17E, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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