Mouse Interleukin 17E Monoclonal Antibody | anti-IL25 antibody

Interleukin 17E (IL-17E)

Cat. Num. AAA600839

• Gene Names: IL25; IL17E
• Reactivity: E Coli, Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified
• Synonyms: Interleukin 17E; Monoclonal Antibody; Interleukin 17E (IL-17E); Anti -Interleukin 17E (IL-17E); anti-IL25 antibody

• Host: Mouse
• Reactivity: E Coli, Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 4H194
• Specificity: Recognizes human Interleukin 17E (IL-17E)
• Purity/Purification: Affinity Purified; Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-IL25 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable use in ELISA and Western Blot.; Dilution: Western Blot: 2ug/ml. In mouse tetses, a ~16kD band is detected.
• Immunogen: Synthetic peptide, aa115-132, corresponding to human IL-17E.
• Positive Control: Mouse testes
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of mouse testis lysate (10ug/lane) using MBS600839.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of adrenal cortex, human formalin fixed paraffin embedded using MBS600839.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed tissue section of mouse liver using MBS600839 at 1:150 dilution. The antibody generated a diffused cytoplasmic staining in the hepatocytes with a very strong signal in the sinusoidal endothelial cells.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of a formalin fixed tissue section of mouse kidney using MBS600839 at 1:150 dilution. The antibody generated a diffused cytoplasmic staining in the tubular and ductal epithelia cells, and a strong positivity in the inter-tubular/ductal blood vessels.)

Related Product Information for anti-IL25 antibody

Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.

Product Categories/Family for anti-IL25 antibody

Antibodies; Abs to Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 27477081
• NCBI GeneID: 64806
• NCBI Accession #: NP_758525.1
• NCBI GenBank Nucleotide #: NM_172314.1
• UniProt Accession #: Q9H293; Q2M3F0; Q8IZV3; Q8WXB0
• Molecular Weight: 20,330 Da
• NCBI Official Full Name: interleukin-25 isoform 2
• NCBI Official Synonym Full Names: interleukin 25
• NCBI Official Symbol: IL25
• NCBI Official Synonym Symbols: IL17E
• NCBI Protein Information: interleukin-25; interleukin-17E
• UniProt Protein Name: Interleukin-25
• Protein Family: Interleukin
• UniProt Gene Name: IL25
• UniProt Synonym Gene Names: IL17E; IL-25; IL-17E
• UniProt Entry Name: IL25_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-17E receptor binding

Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter