Testing Data
(Confocal fluorescent analysis of A431 and HEK293 cells using a 1:100 dilution of MBS601860, Integrin alpha1 and a Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane)
Mouse anti-Human Integrin alpha1L Monoclonal Antibody
Integrin alpha1L
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A Sepharose chromatography.
Synonyms
Integrin alpha1L; Monoclonal Antibody; Anti -Integrin alpha1L; anti-Integrin alpha1L antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q1420
Specificity
Recognizes the I domain (Val151-Ala364) of human a1 integrin (CD49a). Precipitates proteins of Mr 180kD, pI 5.9-6.0 (alpha chain) and Mr 115kD, pI 4.5-5.0 (beta chain) from human lymphocyte lysates. Does not recognize rat recombinant I domain of integrin a1.
Purity/Purification
Affinity Purified
Purified by Protein A Sepharose chromatography.
Purified by Protein A Sepharose chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Applicable Applications for anti-Integrin alpha1L antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry and Inhibition assays.
Dilution: ELISA: 0.5ug/ml
Western Blot: 2ug/ml
Immunohistochemistry: 2ug/ml with paraformaldehyde fixation.
Immunoprecipitation: 1ug/ml
Flow Cytometry: 2ug/ml
Inhibition Assay: 1-5ug/ml. Using activated human lymphocyte binding to laminin, collagen IV and fibronectin.
Dilution: ELISA: 0.5ug/ml
Western Blot: 2ug/ml
Immunohistochemistry: 2ug/ml with paraformaldehyde fixation.
Immunoprecipitation: 1ug/ml
Flow Cytometry: 2ug/ml
Inhibition Assay: 1-5ug/ml. Using activated human lymphocyte binding to laminin, collagen IV and fibronectin.
Immunogen
Human Integrin alpha1L
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Confocal fluorescent analysis of A431 and HEK293 cells using a 1:100 dilution of MBS601860, Integrin alpha1 and a Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane)
Testing Data
(Confocal fluorescent analysis of A431 and HEK293 cells using a 1:100 dilution of MBS601860, Integrin alpha1 and a Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane)
Testing Data
(Huvec (non-reducing) cell lysate was probed with MBS601860, Integrin alpha1 (1:1000 dilution). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.|Arrow indicates target (~110kD))
Testing Data
(Huvec (non-reducing) cell lysate was probed with MBS601860, Integrin alpha1 (1:1000 dilution). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.|Arrow indicates target (~110kD))
Product Categories/Family for anti-Integrin alpha1L antibody
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Product Notes
The Integrin alpha1L (Catalog #AAA601860) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Integrin alpha1L reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha1L can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry and Inhibition assays. Dilution: ELISA: 0.5ug/ml Western Blot: 2ug/ml Immunohistochemistry: 2ug/ml with paraformaldehyde fixation. Immunoprecipitation: 1ug/ml Flow Cytometry: 2ug/ml Inhibition Assay: 1-5ug/ml. Using activated human lymphocyte binding to laminin, collagen IV and fibronectin. Researchers should empirically determine the suitability of the Integrin alpha1L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin alpha1L, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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