Mouse anti-Human Integrin, beta1 Monoclonal Antibody | anti-ITGB1 antibody

Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

Cat. Num. AAA608947

• Gene Names: ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, immunoassay, Radioimmunoassay
• Purity: Affinity Purified; Purified by Protein A chromatography
• Synonyms: Integrin; beta1; Monoclonal Antibody; beta1 (CD29; Very Late Antigen; VLAb1; Platelet gplla); Anti -Integrin; anti-ITGB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q847
• Specificity: Recognizes human Integrin beta 1.
• Purity/Purification: Affinity Purified; Purified by Protein A chromatography
• Form/Format: Supplied as a liquid in 0.02M PBS, pH 7.6, 0.1% sodium azide.

• Applicable Applications for anti-ITGB1 antibody: ELISA (EL/EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Radioimmunoassay (RIA)
• Application Notes: Suitable for use in RIA, Flow Cytometry, ELISA, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Functional blocking.; Dilution: Immunohistochemistry: acetone fixation, not suitable for paraffin embedded.; Immunocytochemistry: paraformaldehyde fixation at <4%
• Immunogen: Human keratinocytes derived from skin.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(Flow Cytometry Analysis:|1x10e6 A431 cells were probed with 4ug of MBS608947 (green) and 2ug of Mouse IgG1 negative control isotype (grey).)

Flow Cytometry (FC/FACS)

(Flow Cytometry Analysis:|1x10e6 HeLa cells were probed with 4ug of MBS608947 (blue) and 2ug of Mouse IgG1 negative control isotype (grey).)

Immunocytochemistry (ICC)

(Immunocytochemistry Analysis:|Confocal fluorescent analysis of HEK293 and A431 cells using a 1:100 dilution of MBS608947 and a  Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane)

Related Product Information for anti-ITGB1 antibody

CD29 is the integrin B1 chain which is expressed as heterodimer non-covalently associated with the integrin subunits 1 to 6 (CD49a to CD49f) and known as very late antigens (VLA). It is also called platelet GPlla. It has been reported as receptor of bronectin. The antigen mediates cellular adhesion primarily to collagen, laminin or fibronectin. It has a broad cellular reactivity but is not expressed by erythrocytes. In normal peripheral blood CD29 blood CD29 is found on approximately 41% of T cells, 40% of CD4+ T lymphocytes, 43% of CD8+ T lymphocytes (1). It is slightly reactive with peripheral B-cells. The co-expression of CD4 and CD29 allows the identi cation of the helper/inducer subpopulation of CD4 lymphocytes.

Product Categories/Family for anti-ITGB1 antibody

Antibodies; Abs to Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 55959577
• NCBI GeneID: 3688
• UniProt Accession #: Q5T3E4; P05556; Q5T3E5; Q5T3E6
• Molecular Weight: 5,325 Da
• NCBI Official Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• NCBI Official Synonym Full Names: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• NCBI Official Symbol: ITGB1
• NCBI Official Synonym Symbols: CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• NCBI Protein Information: integrin beta-1; OTTHUMP00000019420; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide
• UniProt Protein Name: Integrin, beta 1 (Fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Protein Family: Integrin
• UniProt Gene Name: ITGB1
• UniProt Entry Name: Q5T3E4_HUMAN

NCBI Description

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Membrane protein, integral; Cell surface; Cell adhesion; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: cell surface; focal adhesion; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; hemidesmosome; membrane; lamellipodium; cytoplasm; melanosome; plasma membrane; basement membrane; neuromuscular junction; integrin complex; receptor complex; sarcolemma; external side of plasma membrane; cleavage furrow; filopodium

Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; alpha-actinin binding; actin binding; peptide binding; protein kinase binding; viral receptor activity; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding

Biological Process: axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; maternal process involved in pregnancy; axon extension; regulation of cell cycle; positive regulation of apoptosis; cell-matrix adhesion; mesodermal cell differentiation; positive regulation of endocytosis; cell fate specification; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; germ cell migration; tissue homeostasis; visual learning; negative regulation of cell projection organization and biogenesis; response to drug; integrin-mediated signaling pathway; cell migration; regulation of immune response; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; cellular calcium ion homeostasis; formation of radial glial scaffolds; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of neuron differentiation; negative regulation of Rho protein signal transduction; cell migration during sprouting angiogenesis; B cell differentiation; cell-cell adhesion mediated by integrin; stress fiber formation; calcium-independent cell-matrix adhesion; cellular defense response; response to activity; blood coagulation; leukocyte tethering or rolling; homophilic cell adhesion; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; positive regulation of cell migration