SDS-Page
(Figure. SDS-PAGE)
Secreted Frizzled Related Protein 1 Active Protein | SFRP1 active protein
Active Secreted Frizzled Related Protein 1 (SFRP1)
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
(Figure. SDS-PAGE)
SDS-Page
(Figure. SDS-PAGE)
Western Blot (WB)
(Figure. Western Blot)
Western Blot (WB)
(Figure. Western Blot)
Testing Data
(To measuered the ability of SFRP1 on tumor suppression, liver cancer HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of recombinant rat SFRP1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37. Suppression of HepG2 cells after incubation with SFRP1 for 96h observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant SFRP1 for 96h. The result was shown in Figure 3. It was obvious that SFRP1 significantly decreased cell viability of HepG2 cells. (A) HepG2 cells cultured in DMEM, stimulated with 1000ng/mL SFRP1 for 96h; (B) Unstimulated HepG2 cells cultured in DMEM for 96h.Figure. Cell suppresstion of HepG2 cells after stimulated with SFRP1.)
Testing Data
(To measuered the ability of SFRP1 on tumor suppression, liver cancer HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of recombinant rat SFRP1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37. Suppression of HepG2 cells after incubation with SFRP1 for 96h observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant SFRP1 for 96h. The result was shown in Figure 3. It was obvious that SFRP1 significantly decreased cell viability of HepG2 cells. (A) HepG2 cells cultured in DMEM, stimulated with 1000ng/mL SFRP1 for 96h; (B) Unstimulated HepG2 cells cultured in DMEM for 96h.Figure. Cell suppresstion of HepG2 cells after stimulated with SFRP1.)
Biological Activity Test
(Figure. Cell suppresstion of HepG2 cells after stimulated with SFRP1.)
Biological Activity Test
(Figure. Cell suppresstion of HepG2 cells after stimulated with SFRP1.)
NCBI and Uniprot Product Information
Accurate Molecular Mass: 18kDa
NCBI Description
a secreted protein involved in binding Wnt and antagonizing Wnt signaling [RGD, Feb 2006]
Uniprot Description
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
Research Articles on SFRP1
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Product Notes
The SFRP1 sfrp1 (Catalog #AAA2122364) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Val8~Asp153. AAA Biotech's Secreted Frizzled Related Protein 1 can be used in a range of immunoassay formats including, but not limited to, Cell Culture, Activity Assays. Researchers should empirically determine the suitability of the SFRP1 sfrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Secreted Frizzled Related Protein 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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