Human Cysteine dioxygenase type 1 ELISA Kit | CDO1 elisa kit

Human Cysteine dioxygenase type 1 ELISA Kit

Cat. Num. AAA761135

• Reactivity: Human
• Synonyms: Cysteine dioxygenase type 1; Human Cysteine dioxygenase type 1 ELISA Kit; CDO1 (Cysteine dioxygenase type 1)/CDO/CDO-I; CDO1 elisa kit

• Reactivity: Human
• Sequence Length: 200

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.5-32ng/ml
• Sensitivity: 0.191ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CDO1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CDO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CDO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CDO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CDO1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 56786147
• NCBI GeneID: 1036
• NCBI Accession #: NP_001792.2
• NCBI GenBank Nucleotide #: NM_001801.2
• Molecular Weight: 22,972 Da
• NCBI Official Full Name: cysteine dioxygenase type 1
• NCBI Official Synonym Full Names: cysteine dioxygenase type 1
• NCBI Official Symbol: CDO1
• NCBI Protein Information: cysteine dioxygenase type 1
• UniProt Protein Name: Cysteine dioxygenase type 1
• Protein Family: Cysteine dioxygenase
• UniProt Gene Name: CDO1
• UniProt Synonym Gene Names: CDO; CDO-I
• UniProt Entry Name: CDO1_HUMAN

Uniprot Description

CDO1: Initiates several important metabolic pathways related to pyruvate and several sulfurate compounds including sulfate, hypotaurine and taurine. Critical regulator of cellular cysteine concentrations. Has an important role in maintaining the hepatic concentation of intracellular free cysteine within a proper narrow range. Belongs to the cysteine dioxygenase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; EC 1.13.11.20; Oxidoreductase; Other Amino Acids Metabolism - taurine and hypotaurine

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: cytosol

Molecular Function: cysteine dioxygenase activity; ferrous iron binding

Biological Process: cysteine metabolic process; inflammatory response; L-cysteine catabolic process; lactation; response to amino acid stimulus; response to cAMP; response to ethanol; response to glucagon stimulus; response to glucocorticoid stimulus; sulfur amino acid biosynthetic process; sulfur amino acid catabolic process; sulfur amino acid metabolic process; taurine biosynthetic process