Mouse anti-Human, Mouse IMP3 Monoclonal Antibody | anti-IMP3 antibody

IMP3 Monoclonal Antibody

Cat. Num. AAA3014486

• Gene Names: IGF2BP3; KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: ProG purified.
• Synonyms: IMP3; Monoclonal Antibody; IMP3 Monoclonal Antibody; IGF2 mRNA-binding protein 3; IMP-3; hKOC; IGF2BP3; KOC1; VICKZ3; Cancer/testis antigen 98; CT98; DKFZp686F1078; IF2B3; IGF II mRNA binding protein 3; IGF-II mRNA-binding protein 3; IGF2 mRNA binding protein 3; IMP 3; Insulin like growth factor 2 mRNA binding protein 3; Insulin-like growth factor 2 mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; KH domain-containing protein overexpressed in cancer; KOC 1; VICKZ 3; VICKZ family member 3; anti-IMP3 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: IMP3 monoclonal antibody detects endogenous levels of IMP3 protein.
• Purity/Purification: ProG purified.
• Form/Format: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 579

• Applicable Applications for anti-IMP3 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1,000; FC/FACS: 1:50-1:100
• Immunogen: Recombinant protein within human IMP3 aa 350-650.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of IMP3 on 293T cell lysate using anti-IMP3 antibody at 1/2,000 dilution.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of PANC-1 cells with IMP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.)

Related Product Information for anti-IMP3 antibody

RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.

NCBI and Uniprot Product Information

• NCBI GI #: 30795212
• NCBI GeneID: 10643
• NCBI Accession #: NP_006538.2
• NCBI GenBank Nucleotide #: NP_006538.2
• UniProt Accession #: O00425; Q9CPN8
• Molecular Weight: 64kDa
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 3
• NCBI Official Synonym Full Names: insulin like growth factor 2 mRNA binding protein 3
• NCBI Official Symbol: IGF2BP3
• NCBI Official Synonym Symbols: KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 3
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 3
• Protein Family: U3 small nucleolar ribonucleoprotein protein
• UniProt Gene Name: IGF2BP3
• UniProt Synonym Gene Names: IMP3; KOC1; VICKZ3; IGF2 mRNA-binding protein 3; IMP-3; hKOC
• UniProt Entry Name: IF2B3_HUMAN

NCBI Description

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF2BP3: RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. Belongs to the RRM IMP/VICKZ family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; Translation

Chromosomal Location of Human Ortholog: 7p11

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: anatomical structure morphogenesis; mRNA transport; translation; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process