Rabbit anti-Human, Mouse IGF2BP3 Polyclonal Antibody | anti-IGF2BP3 antibody

IGF2BP3 Antibody

Cat. Num. AAA7048444

• Gene Names: IGF2BP3; KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: IGF2BP3; Polyclonal Antibody; IGF2BP3 Antibody; Insulin-like growth factor 2 mRNA-binding protein 3IGF2 mRNA-binding protein 3IMP-3; IGF-II mRNA-binding protein 3; KH domain-containing protein overexpressed in cancer; hKOC; VICKZ family member 3; IMP3; KOC1; VICKZ3; anti-IGF2BP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 579

• Applicable Applications for anti-IGF2BP3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Insulin-like growth factor 2 mRNA-binding protein 3 protein (1-100AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-100766 / sc-292451 / sc-47892 / sc-47893
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Hela whole cell lysate,K562 whole cell lysate,HEK293 whole cell lysate,Mouse brain tissueAll lanes: IGF2BP3 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,22 kDaObserved band size: 64,50 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7048444 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric cancer using MBS7048444 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells using MBS7048444 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-IGF2BP3 antibody

RNA-binding factor that may recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.

NCBI and Uniprot Product Information

• NCBI GI #: 30795212
• NCBI GeneID: 10643
• NCBI Accession #: NP_006538.2
• NCBI GenBank Nucleotide #: NM_006547.2
• UniProt Accession #: O00425; Q63HM0; Q6MZZ2; Q86VB1; A0A4Z5
• Molecular Weight: 21,630 Da
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 3
• NCBI Official Synonym Full Names: insulin like growth factor 2 mRNA binding protein 3
• NCBI Official Symbol: IGF2BP3
• NCBI Official Synonym Symbols: KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 3
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 3
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP3
• UniProt Synonym Gene Names: IMP3; KOC1; VICKZ3; IGF2 mRNA-binding protein 3; IMP-3; hKOC
• UniProt Entry Name: IF2B3_HUMAN

NCBI Description

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF2BP3: RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. Belongs to the RRM IMP/VICKZ family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; Translation

Chromosomal Location of Human Ortholog: 7p11

Cellular Component: cytoplasm; cytosol

Molecular Function: mRNA 3'-UTR binding; mRNA 5'-UTR binding; protein binding; RNA binding; translation regulator activity

Biological Process: anatomical structure morphogenesis; gene expression; negative regulation of translation; regulation of cytokine biosynthetic process; translation