Western Blot (WB)
(Western Blot: Sample: Rabbit Serum Primary Ab: 1ug/ml Mouse Anti-Rabbit IgG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Mouse anti-Rabbit Immunoglobulin G Monoclonal Antibody | anti-IgG antibody
Monoclonal Antibody to Immunoglobulin G (IgG)
Reactivity
Rabbit
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Immunoglobulin G; Monoclonal Antibody; Monoclonal Antibody to Immunoglobulin G (IgG); anti-IgG antibody
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C3
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IgG antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2 ug/mL;1:500-2000
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-204g/mL;1:50-200
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-204g/mL;1:50-200
Optimal working dilutions must be determined by end user.
Organism Species
Oryctolagus cuniculus (Rabbit)
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Native IgG
Cross Reactivity
Rabbit
Conjugate
No Conjugate
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043751
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Rabbit Serum Primary Ab: 1ug/ml Mouse Anti-Rabbit IgG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rabbit Serum Primary Ab: 1ug/ml Mouse Anti-Rabbit IgG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rabbit Serum.)
Western Blot (WB)
(Western Blot: Sample: Rabbit Serum.)
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Product Notes
The IgG (Catalog #AAA2025784) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Immunoglobulin G (IgG) reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Immunoglobulin G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL;1:500-2000 Immunohistochemistry: 5-20 ug/mL;1:50-200 Immunocytochemistry: 5-204g/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Immunoglobulin G, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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