Western Blot (WB)
(Western Blot: Sample: Recombinant IFNg, Rabbit.)
Guinea pig anti-Rabbit Interferon Gamma (IFNg) Polyclonal Antibody | anti-IFNg antibody
Polyclonal Antibody to Interferon Gamma (IFNg)
Reactivity
Rabbit
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Interferon Gamma (IFNg); Polyclonal Antibody; Polyclonal Antibody to Interferon Gamma (IFNg); anti-IFNg antibody
Host
Guinea pig
Reactivity
Rabbit
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% lycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-IFNg antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Oryctolagus cuniculus (Rabbit)
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Rabbit
Immunogen
Recombinant IFNg (Gln24~Tyr167) expressed in E.coli (MBS2012242)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 8h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 8h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant IFNg, Rabbit.)
Western Blot (WB)
(Western Blot: Sample: Recombinant IFNg, Rabbit.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rabbit Kidney Tissue; Primary Ab: 20ug/ml Cavia Anti-Rabbit IFNg Antibody Second Ab: 2ug/mL HRPLinked Rabbit Anti-Cavia IgG Polyclonal Antibody (Catalog: MBS2086026))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rabbit Kidney Tissue; Primary Ab: 20ug/ml Cavia Anti-Rabbit IFNg Antibody Second Ab: 2ug/mL HRPLinked Rabbit Anti-Cavia IgG Polyclonal Antibody (Catalog: MBS2086026))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interferon gamma
NCBI Official Symbol
IFNG
NCBI Protein Information
interferon gamma
UniProt Protein Name
Interferon gamma
Protein Family
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
Uniprot Description
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
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Product Notes
The IFNg ifng (Catalog #AAA2003385) is an Antibody produced from Guinea pig and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interferon Gamma (IFNg) reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon Gamma (IFNg) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IFNg ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon Gamma (IFNg), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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