Testing Data
(Published customer image: Main cell population producing IL-12 in response to R-848.Neonatal MLN cells were cultured in vitro with or without 0.5 ug/ml R-848 and, at various time points, supernatants were harvested and ELISA was carried out for IL-12. Mean +/- SEM IL-12 secretion is shown for each time point (A). Neonatal MLN cells were cultured in vitro with (black bars) or without (hatched bars) 0.5 ug/ml R-848 for 8 h, with brefeldin A added for the last 5 h. Cells were harvested for IL-12 intracellular staining combined with labelling of CD11b, CD14, MHC class II and CD205. Three independent experiments were carried out and the data shown are the mean proportions of IL-12+ cells after gating on CD11b+, CD14+, MHC class II+ or CD205+ MLN cells (B).From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
Mouse IL-12 Monoclonal Antibody | anti-IL12B antibody
MOUSE ANTI BOVINE INTERLEUKIN-12:Preservative Free
Purified IgG - liquid
ELISA: Minimum Dilution: 1/100; Maximum Dilution: 1/1000
Flow Cytometry: Maximum Dilution: 1/1000; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Functional Assays: Maximum Dilution: 1/100
Fusion Partners
Shelf Life: 18 months from date of despatch.
Testing Data
(Published customer image: Main cell population producing IL-12 in response to R-848.Neonatal MLN cells were cultured in vitro with or without 0.5 ug/ml R-848 and, at various time points, supernatants were harvested and ELISA was carried out for IL-12. Mean +/- SEM IL-12 secretion is shown for each time point (A). Neonatal MLN cells were cultured in vitro with (black bars) or without (hatched bars) 0.5 ug/ml R-848 for 8 h, with brefeldin A added for the last 5 h. Cells were harvested for IL-12 intracellular staining combined with labelling of CD11b, CD14, MHC class II and CD205. Three independent experiments were carried out and the data shown are the mean proportions of IL-12+ cells after gating on CD11b+, CD14+, MHC class II+ or CD205+ MLN cells (B).From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
Testing Data
(Published customer image: Main cell population producing IL-12 in response to R-848.Neonatal MLN cells were cultured in vitro with or without 0.5 ug/ml R-848 and, at various time points, supernatants were harvested and ELISA was carried out for IL-12. Mean +/- SEM IL-12 secretion is shown for each time point (A). Neonatal MLN cells were cultured in vitro with (black bars) or without (hatched bars) 0.5 ug/ml R-848 for 8 h, with brefeldin A added for the last 5 h. Cells were harvested for IL-12 intracellular staining combined with labelling of CD11b, CD14, MHC class II and CD205. Three independent experiments were carried out and the data shown are the mean proportions of IL-12+ cells after gating on CD11b+, CD14+, MHC class II+ or CD205+ MLN cells (B).From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
Testing Data
(Published customer image: MLN and spleen cell IL-12 responses to R-848 stimulation as a function of age. MLN and spleen cells from 6- to 14-day-old neonates (closed circles), 20-day-old lambs (closed diamonds) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 ug/ml R-848. At the end of the culture period, supernatants were harvested and ELISA carried out to assess IL-12 secretion by MLN cells (A) or spleen cells (B). Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates, 20-day-old lambs and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
Testing Data
(Published customer image: MLN and spleen cell IL-12 responses to R-848 stimulation as a function of age. MLN and spleen cells from 6- to 14-day-old neonates (closed circles), 20-day-old lambs (closed diamonds) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 ug/ml R-848. At the end of the culture period, supernatants were harvested and ELISA carried out to assess IL-12 secretion by MLN cells (A) or spleen cells (B). Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates, 20-day-old lambs and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
Testing Data
(Published customer image: Comparative IL-12 and IFN&gamma responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 ug/ml polyI:C, 0.5 ug/ml R-848 or 5 ug/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) and IFN? (B) secretion. Non-specific cell stimulation was also carried out with 50 ng/ml PMA combined with 500 ng/ml ionomycin, with the supernatants assayed for IFN? detection 48 h later (C). Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
Testing Data
(Published customer image: Comparative IL-12 and IFN&gamma responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 ug/ml polyI:C, 0.5 ug/ml R-848 or 5 ug/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) and IFN? (B) secretion. Non-specific cell stimulation was also carried out with 50 ng/ml PMA combined with 500 ng/ml ionomycin, with the supernatants assayed for IFN? detection 48 h later (C). Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
Testing Data
(Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.)
Testing Data
(Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.)
Testing Data
(Published customer image: Roles of TGFbeta1 and IL-10 in regulating IL-12 responses to R-848. MLN cells from neonates (closed circles) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 ug/ml R-848, in the presence of rhTGFbeta1 (n = 7) (A) or rovIL-10 (n = 5) (B). Supernatants were harvested and ELISA was carried out to assess IL-12 secretion. The mean +/- SEM level of IL-12 secretion is shown (A, B). RNA was extracted and purified from freshly isolated MLN cells. IL-10 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (C). MLN cells from neonates (closed circles) and adults (open squares) were stimulated in vitro for 48 h with or without 0.5 ug/ml R-848. Supernatants were harvested and ELISA carried out to assess IL-10 secretion. Medians are indicated. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: **p=0.001 (D). RNA was extracted and purified from freshly isolated MLN cells. Foxp3 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (E). Comparison of the IL-12 response of neonate MLN cells to R-848 stimulation in culture medium supplemented with 10%FCS, 10% neonate autologous plasma or 10% adult plasma. Paired t-test between neonate and adult plasma were non-significant (F).From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
Testing Data
(Published customer image: Roles of TGFbeta1 and IL-10 in regulating IL-12 responses to R-848. MLN cells from neonates (closed circles) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 ug/ml R-848, in the presence of rhTGFbeta1 (n = 7) (A) or rovIL-10 (n = 5) (B). Supernatants were harvested and ELISA was carried out to assess IL-12 secretion. The mean +/- SEM level of IL-12 secretion is shown (A, B). RNA was extracted and purified from freshly isolated MLN cells. IL-10 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (C). MLN cells from neonates (closed circles) and adults (open squares) were stimulated in vitro for 48 h with or without 0.5 ug/ml R-848. Supernatants were harvested and ELISA carried out to assess IL-10 secretion. Medians are indicated. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: **p=0.001 (D). RNA was extracted and purified from freshly isolated MLN cells. Foxp3 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (E). Comparison of the IL-12 response of neonate MLN cells to R-848 stimulation in culture medium supplemented with 10%FCS, 10% neonate autologous plasma or 10% adult plasma. Paired t-test between neonate and adult plasma were non-significant (F).From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)
NCBI and Uniprot Product Information
Uniprot Description
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Research Articles on IL12B
Similar Products
Product Notes
The IL12B il12b (Catalog #AAA215578) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL-12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS)*, Functional Assays (FN). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells of 100ul. ELISA: Minimum Dilution: 1/100; Maximum Dilution: 1/1000 Flow Cytometry: Maximum Dilution: 1/1000; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Functional Assays: Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the IL12B il12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
