Mouse anti-Human Interleukin-12 p40 Monoclonal Antibody | anti-IL-12p40 antibody

Mouse anti Human Interleukin-12 p40

Cat. Num. AAA226164

• Gene Names: IL12B; CLMF; NKSF; CLMF2; NKSF2; IL-12B
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: Interleukin-12 p40; Monoclonal Antibody; Mouse anti Human Interleukin-12 p40; IL-12 p40 antibody | D14-5G11; anti-IL-12p40 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: D14-5G11
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)

• Applicable Applications for anti-IL-12p40 antibody: ELISA (EIA)
• Application Notes: ELISA: 1.0ug/ml
• Immunogen: E Coli derived recombinant human full length interleukin-12 p40 (Ile23-Ser328)
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human IL-12 p40 using Mouse anti Human interleukin-12 p40 (MBS226164) as a capture reagent and biotinylated Mouse anti Human interleukin-12 p40 as a detection reagent with recombinant human IL-12 p40 as the antigen. D)

Related Product Information for anti-IL-12p40 antibody

Mouse anti Human Interleukin-12 p40 antibody, clone D14-5G11, recognizes interleukin-12 p40 (IL-12 p40) also known as interleukin-12 subunit beta and cytotoxic lymphocyte maturation factor 40kDa subunit. IL-12 is a heterodimeric cytokine composed of 2 subunits, a 35kDa subunit (p35) and a 40kDa subunit (p40). The IL-12 p40 subunit is largely expressed by dendritic cells, monocytes, macrophages, neutrophils, B-lymphocytes and keratinocytes (Airoldi et al. 2002). It is able to form a homodimer, which was believed to be inactive. However it has subsequently been demonstrated to have proinflammatory properties through its induction of inducible nitric oxide synthase in microglia (Pahan et al. 2001, Jana et al. 2009). IL-12 p40 coupled to the protein p19 forms the heterodimer IL-23 (p19-p40), when this formation takes place in activated dentritic cells the p19-p40 binds to its receptor IL-12Rbeta1 to activate the STAT4 signaling pathway. This results in the stimulation of IFNgamma production along with proliferation of PHA blast T cells (Airoldi et al. 2002).

The purified Mouse anti Human interleukin-12 p40 antibody, clone D14-5G11 can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-12 p40 antibody, clone M18 as the detection antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 24497438
• NCBI GeneID: 3593
• NCBI Accession #: NP_002178.2
• NCBI GenBank Nucleotide #: NM_002187.2
• UniProt Accession #: P29460
• Molecular Weight: 328
• NCBI Official Full Name: interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• NCBI Official Symbol: IL12B
• NCBI Official Synonym Symbols: CLMF; NKSF; CLMF2; NKSF2; IL-12B
• NCBI Protein Information: interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor,
• UniProt Protein Name: Interleukin-12 subunit beta
• UniProt Gene Name: IL12B
• UniProt Synonym Gene Names: NKSF2; IL-12B; CLMF p40; NKSF2
• UniProt Entry Name: IL12B_HUMAN