Rat anti-Mouse IL-10 Monoclonal Antibody | anti-IL-10 antibody
Rat Anti-Mouse IL-10
Gene Names
Il10; CSIF; Il-10
Reactivity
Mouse
Purity
Protein G chromatography
Synonyms
IL-10; Monoclonal Antibody; Rat Anti-Mouse IL-10; Anti-murine IL-10; anti-IL-10 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Protein G chromatography
Form/Format
Lyophilized
Sequence Length
178
Application Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/ml), a concentration of 3.0-5.0 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used in conjunction with compatible secondary reagents. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Antibody Generation
Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-10 as the immunizing antigen. This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.
Buffer
PBS, pH 7.2
Related Product Information for anti-IL-10 antibody
Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper (Th) 2 clones that inhibited cytokine production by Th1 clones. The human ortholog of mouse IL-10 was subsequently cloned by cross-hybridization. Human and mouse IL-10 are 81% identical at the nucleotide and amino acid level. IL-10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. A number of viruses, including Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV), also encode viral members of the IL-10 family. Human IL-10 has cross-species actvity and is active on mouse cells. Mouse IL-10 is species-specific and does not act on human cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,641 Da
NCBI Official Full Name
interleukin-10
NCBI Official Synonym Full Names
interleukin 10
NCBI Official Symbol
Il10
NCBI Official Synonym Symbols
CSIF; Il-10
NCBI Protein Information
interleukin-10; cytokine synthesis inhibitory factor
UniProt Protein Name
Interleukin-10
UniProt Gene Name
Il10
UniProt Synonym Gene Names
Il-10; IL-10; CSIF
UniProt Entry Name
IL10_MOUSE
Uniprot Description
Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subunit structure: Homodimer
By similarity.
Subcellular location: Secreted.
Sequence similarities: Belongs to the IL-10 family.
Research Articles on IL-10
Similar Products
Product Notes
The IL-10 il10 (Catalog #AAA690328) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IL-10 reacts with Mouse and may cross-react with other species as described in the data sheet. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/ml), a concentration of 3.0-5.0 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used in conjunction with compatible secondary reagents. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. Researchers should empirically determine the suitability of the IL-10 il10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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