Mouse anti-Human Ikke/Ikki/TBK1 Monoclonal Antibody | anti-Ikke/Ikki/TBK1 antibody

Monoclonal Antibody to Ikke/Ikki/TBK1 (Clone: ABM13C7)

Cat. Num. AAA668410

• Gene Names: IKBKE; IKKE; IKKI; IKK-E; IKK-i
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified; Protein G Chromatography
• Synonyms: Ikke/Ikki/TBK1; Monoclonal Antibody; Monoclonal Antibody to Ikke/Ikki/TBK1 (Clone: ABM13C7); IKBKE; IKKE; IKKI; KIAA0151; Inducible I kappa-B kinase; Antibody to Ikke/Ikki/TBK1; Anti-Ikke/Ikki/TBK1 Antibody; Ikke/Ikki/TBK1 Antibody; anti-Ikke/Ikki/TBK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone Number: ABM13C7
• Purity/Purification: Purified; Protein G Chromatography
• Form/Format: 25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
• Sequence Length: 716

• Applicable Applications for anti-Ikke/Ikki/TBK1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB: 2-4 ug/ml; IHC: 10-20 ug/ml
• Immunogen Information: A partial length recombinant Ikke protein (amino acids 2-203) was used as the immunogen for this antibody.
• Antigen: Ikke/Ikki/TBK1
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.; For long-term storage, store at -20 degree C.; Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Fig-1: Western blot analysis of IKKe. Anti-Ikke antibody (Clone: ABM13C7) was tested at 2 ug/ml on Jurkat, NIH 3T3 and HepG2 lysates.)

Immunohistochemistry (IHC)

(Fig-2: Immunohistochemical analysis of Ikke in Thyroid cancer tissue using Ikke antibody (Clone: ABM13C7) at 10 ?g/ml.)

Related Product Information for anti-Ikke/Ikki/TBK1 antibody

Ikke is a member of the IKK (IKappaB kinase) family that has been identified as an oncogenic protein and found to be up-regulated in breast cancer, ovarian cancer and prostate cancer. The Ikke protein has 33% and 31% identity at the amino acid level with Ikka and Ikkb, respectively and is primarily activated by interferon and mediates interferon signaling. The activated Ikke stimulates the transcription factor interferon regulatory factors 3 and phosphorylates signal transducer and activator of transcription. Ikke also shares some function with Ikka and Ikkb to activate NF- KappaB pathway by phosphorylation and degradation of IKappaBalpha. However, Ikke mainly mediates NF-KappaB activation induced by interferon, phorbol 12-myristate 13-acetate or the T-cell receptor, but not by tumor necrosis factor and interleukin 1, which activate Ikka and Ikkb. Ikke is predominantly expressed and active in peripheral blood leukocytes, pancreas, thymus, and spleen and contributes to IRF as well as NF-KappaB activation.

Product Categories/Family for anti-Ikke/Ikki/TBK1 antibody

Antibodies; NF-kB Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 14548079
• NCBI GeneID: 9641
• UniProt Accession #: Q14164; Q3B754; Q3KR43; Q5JTS6; D3DT78
• Molecular Weight: 70,816 Da
• NCBI Official Full Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon
• NCBI Official Synonym Full Names: inhibitor of nuclear factor kappa B kinase subunit epsilon
• NCBI Official Symbol: IKBKE
• NCBI Official Synonym Symbols: IKKE; IKKI; IKK-E; IKK-i
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit epsilon
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon
• UniProt Gene Name: IKBKE
• UniProt Synonym Gene Names: IKKE; IKKI; KIAA0151; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; IKK-i

NCBI Description

IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]

Uniprot Description

IKKE: a oncogenic kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Plays a pivotal role in coordinating the activation of IRF3 and NF-kappaB in the innate immune response. Its redistribution to PML nuclear bodies upon DNA damage is TOPORS-dependent. Is a breast cancer oncogene that exerts its oncogenic properties through cRel.

Protein type: EC 2.7.11.10; IKK family; Inhibitor; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytoplasm; cytosol; endosome membrane; mitochondrial membrane; nucleus; PML body

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; protein kinase activity; ubiquitin protein ligase binding

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; innate immune response; negative regulation of interferon type I production; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein amino acid phosphorylation; protein homooligomerization