Rabbit anti-Human IKBKE Monoclonal Antibody | anti-IKBKE antibody

IKBKE Antibody

Cat. Num. AAA9436448

• Gene Names: IKBKE; IKKE; IKKI; IKK-E; IKK-i
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: IKBKE; Monoclonal Antibody; IKBKE Antibody; I kappa B kinase epsilon antibody; I-kappa-B kinase epsilon antibody; IkBKE antibody; IKK related kinase epsilon antibody; IKK-E antibody; IKK-epsilon antibody; IKK-i antibody; IKKE antibody; IKKE_HUMAN antibody; IKKepsilon antibody; IKKI antibody; Inducible I kappa B kinase antibody; Inducible I kappa-B kinase antibody; Inducible IkappaB kinase antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells; kinase of; epsilon antibody; Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody; KIAA0151 antibody; MGC125294 antibody; MGC125295 antibody; MGC125297 antibody; anti-IKBKE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: JU06-72
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-IKBKE antibody: Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of IKBKE on different cell lysates using anti-IKBKE antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Raji Lane 3: MCF-7 Lane 4: Jurkat)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IKBKE antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining IKBKE in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining IKBKE in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining IKBKE in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-IKBKE antibody

The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkappaB-alpha and specifically phosphorylates IkappaB-alpha on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKalpha and IKKbeta . IKK-i is primarily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFalpha, IL-1 and IL-6. Overexpression of IKK-i has been shown to result in phosphorylation of IkappaBalpha on Ser 32 and Ser 36, and in NFkappaB activation, suggesting that IKK-i may act as an IkappaB kinase in the immune system.

Product Categories/Family for anti-IKBKE antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 14548079
• NCBI GeneID: 9641
• UniProt Accession #: Q14164; Q3B754; Q3KR43; Q5JTS6; D3DT78
• Molecular Weight: 716
• NCBI Official Full Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• NCBI Official Symbol: IKBKE
• NCBI Official Synonym Symbols: IKKE; IKKI; IKK-E; IKK-i
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit epsilon; IKK-epsilon; I-kappa-B kinase epsilon; inducible IkappaB kinase; IKK-related kinase epsilon; inducible I kappa-B kinase
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: IKBKE
• UniProt Synonym Gene Names: IKKE; IKKI; KIAA0151; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; IKK-i
• UniProt Entry Name: IKKE_HUMAN

NCBI Description

IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]

Uniprot Description

IKKE: a oncogenic kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Plays a pivotal role in coordinating the activation of IRF3 and NF-kappaB in the innate immune response. Its redistribution to PML nuclear bodies upon DNA damage is TOPORS-dependent. Is a breast cancer oncogene that exerts its oncogenic properties through cRel.

Protein type: Inhibitor; Kinase, protein; EC 2.7.11.10; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; PML body; cytoplasm; mitochondrial membrane; endosome membrane; nucleus; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; IkappaB kinase activity; ATP binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; DNA damage response, signal transduction resulting in induction of apoptosis; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; toll-like receptor 3 signaling pathway; I-kappaB phosphorylation; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; protein amino acid phosphorylation