IKKe recombinant protein

Recombinant IKKe protein

Cat. Num. AAA389174

• Gene Names: IKBKE; IKKE; IKKI; IKK-E; IKK-i; KIAA0151; MGC125294; MGC125295; MGC125297
• Synonyms: IKKe; Recombinant IKKe protein; IKBKE; Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Epsilon; IKKe recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant IKKe protein is supplied at a concentration of 0.3ug/ul in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Kinase Activity Assay Conditions: 1uM STK S3 substrate was incubated with different concentrations of IKKe protein in 10ul reaction system containing 1×Enzymatic Buffer, 5mM MgCl2, 1mM DTT and 100uM ATP for 1 hour. The 10ul detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer, were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant IKKe protein 10% SDS-PAGE Coomassie staining MW: 81.7 kDa Purity: >90% )

Testing Data

(HTRF assay for IKKe activity 1 uM STK S3 substrate was incubated with different concentrations of IKKe protein in reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)

Testing Data

(HTRF assay for IKKe activity 1 uM STK S3 substrate was incubated with different concentrations of IKKe protein in reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)

Related Product Information for IKKe recombinant protein

Short Description: Recombinant human IKKe protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_054721.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 81.7 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: IKKe (Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Epsilon, also known as IKBKE), is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. It's a serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. It also involves in TNFa and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, IKKe associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNb. In order to establish such an antiviral state, IKKe forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKKe-containing-complexes. It can be activated by polyubiquitination in response to TNF&alpha and interleukin-1, regulate the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. It can phosphorylate inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, IKKe is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at Ser-708. Phosphorylation of STAT1 at Ser- 708 seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses.

Product Categories/Family for IKKe recombinant protein

Recombinant Proteins; Kinase; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 7661946
• NCBI GeneID: 9641
• NCBI Accession #: NP_054721.1
• NCBI GenBank Nucleotide #: NM_014002.3
• UniProt Accession #: Q14164; Q3KR43; Q5JTS6; D3DT78; Q3B754
• Molecular Weight: 80,462 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 1
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• NCBI Official Symbol: IKBKE
• NCBI Official Synonym Symbols: IKKE; IKKI; IKK-E; IKK-i; KIAA0151; MGC125294; MGC125295; MGC125297
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit epsilon; IKK-epsilon; OTTHUMP00000034540; I-kappa-B kinase epsilon; inducible IkappaB kinase; IKK-related kinase epsilon; inducible I kappa-B kinase
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon
• UniProt Gene Name: IKBKE
• UniProt Synonym Gene Names: IKKE; IKKI; KIAA0151; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; IKK-i
• UniProt Entry Name: IKKE_HUMAN

Uniprot Description

IKKE: a oncogenic kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Plays a pivotal role in coordinating the activation of IRF3 and NF-kappaB in the innate immune response. Its redistribution to PML nuclear bodies upon DNA damage is TOPORS-dependent. Is a breast cancer oncogene that exerts its oncogenic properties through cRel.

Protein type: Inhibitor; Kinase, protein; EC 2.7.11.10; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; PML body; cytoplasm; mitochondrial membrane; endosome membrane; nucleus; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; IkappaB kinase activity; ATP binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; DNA damage response, signal transduction resulting in induction of apoptosis; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; toll-like receptor 3 signaling pathway; I-kappaB phosphorylation; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; protein amino acid phosphorylation