Western Blot (WB)
(Plate was coated with human IgE at 1.25 ug/ml in PBS, and then incubated with anti-human IgE antibody (1497CT272.23.66) from 0.004 ug/ml to 1 ug/ml. The secondary antibody, HRP conjugated goat anti-mouse antibody,were used at 1:10000. )
Mouse anti-Human IgE Monoclonal Antibody | anti-IgE antibody
Mouse Anti-Human IgE Antibody
Reactivity
Human
Applications
ELISA
Synonyms
IgE; Monoclonal Antibody; Mouse Anti-Human IgE Antibody; Mouse Monoclonal Antibody (Mab); anti-IgE antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
1497CT272.23.66
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applicable Applications for anti-IgE antibody
ELISA (EIA)
Application Notes
E ~~ 1:5000
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Plate was coated with human IgE at 1.25 ug/ml in PBS, and then incubated with anti-human IgE antibody (1497CT272.23.66) from 0.004 ug/ml to 1 ug/ml. The secondary antibody, HRP conjugated goat anti-mouse antibody,were used at 1:10000. )
Western Blot (WB)
(Plate was coated with human IgE at 1.25 ug/ml in PBS, and then incubated with anti-human IgE antibody (1497CT272.23.66) from 0.004 ug/ml to 1 ug/ml. The secondary antibody, HRP conjugated goat anti-mouse antibody,were used at 1:10000. )
Product Categories/Family for anti-IgE antibody
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Product Notes
The IgE (Catalog #AAA9215376) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IgE Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). E ~~ 1:5000. Researchers should empirically determine the suitability of the IgE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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