Immunohistochemistry (IHC) - Paraffin
(Dilution: IHC-P - 1:25Immunohistochemical analysis of PDL-2 in untransfected(left) or transfected(right) with 293T cell sections. Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Mouse anti-Human PDCD1LG2 Monoclonal Antibody | anti-PDCD1LG2 antibody
PDCD1LG2 Antibody (N-term)
Immunohistochemistry (IHC) - Paraffin
(Dilution: IHC-P - 1:25Immunohistochemical analysis of PDL-2 in untransfected(left) or transfected(right) with 293T cell sections. Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC) - Paraffin
(Dilution: IHC-P - 1:25Immunohistochemical analysis of PDL-2 in untransfected(left) or transfected(right) with 293T cell sections. Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Western Blot (WB)
(Dilution: WB - 1:8000All lanes: Anti-PDCD1LG2 Antibody (N-term) at 1:8000 dilution Lane 1: Non-transfected 293T whole cell lysate Lane 2: Transfected PD-L2-transfected 293T whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Dilution: WB - 1:8000All lanes: Anti-PDCD1LG2 Antibody (N-term) at 1:8000 dilution Lane 1: Non-transfected 293T whole cell lysate Lane 2: Transfected PD-L2-transfected 293T whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
Uniprot Description
PDCD1LG2: Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 9p24.2
Cellular Component: plasma membrane
Biological Process: negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; T cell costimulation
Research Articles on PDCD1LG2
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Product Notes
The PDCD1LG2 pdcd1lg2 (Catalog #AAA9231333) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PDCD1LG2 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDCD1LG2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the PDCD1LG2 pdcd1lg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDCD1LG2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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