Sandwich ELISA (EIA)
(Plate was coated with BSA-Beta-Estradiol at 1.25 ug/ml in PBS, and then incubated with anti-Beta-Estradiol monoclonal antibody (1977CT128.130.83) from 0.004 ug/ml to 2 ug/ml. The secondary antibody, HRP conjugated goat anti-mouse antibody,were used at 1:10000 dilution. The antibody inhibitory concentration(IC50) of beta-Estradiol was 33.47ng/mL)
Mouse Beta-Estradiol Monoclonal Antibody
Anti Beta-Estradiol Monoclonal Antibody (1977CT128.130.83)
Applications
ELISA
Synonyms
Beta-Estradiol; Monoclonal Antibody; Anti Beta-Estradiol Monoclonal Antibody (1977CT128.130.83); Anti beta-Estradiol monoclonal antibody; anti-Beta-Estradiol antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,kappa
Specificity
Mouse monoclonal antibody raised against Trastuzumab.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applicable Applications for anti-Beta-Estradiol antibody
ELISA (EIA)
Application Notes
Sandwich ELISA
Antigen Source
Estradiol (E2), also spelled oestradiol, is a steroid, an estrogen, and the primary female sex hormone. It is named for and is important in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is essential for the development and maintenance of female reproductive tissues such as the breasts, uterus, and vagina during puberty, adulthood, and pregnancy,but it also has important effects in many other tissues, including bone, fat, skin, liver, and the brain. While estrogen levels in men are lower compared to those in women, estrogens have essential functions in men, as well. It is found in most vertebrates and crustaceans, insects, fish, and other animal species.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Sandwich ELISA (EIA)
(Plate was coated with BSA-Beta-Estradiol at 1.25 ug/ml in PBS, and then incubated with anti-Beta-Estradiol monoclonal antibody (1977CT128.130.83) from 0.004 ug/ml to 2 ug/ml. The secondary antibody, HRP conjugated goat anti-mouse antibody,were used at 1:10000 dilution. The antibody inhibitory concentration(IC50) of beta-Estradiol was 33.47ng/mL)
Sandwich ELISA (EIA)
(Plate was coated with BSA-Beta-Estradiol at 1.25 ug/ml in PBS, and then incubated with anti-Beta-Estradiol monoclonal antibody (1977CT128.130.83) from 0.004 ug/ml to 2 ug/ml. The secondary antibody, HRP conjugated goat anti-mouse antibody,were used at 1:10000 dilution. The antibody inhibitory concentration(IC50) of beta-Estradiol was 33.47ng/mL)
Related Product Information for anti-Beta-Estradiol antibody
Purified Mouse Monoclonal Antibody (Mab)
Similar Products
Product Notes
The Beta-Estradiol (Catalog #AAA9232240) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Beta-Estradiol can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Sandwich ELISA. Researchers should empirically determine the suitability of the Beta-Estradiol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-Estradiol, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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