Immunohistochemistry (IHC)
(Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Mouse anti-Human HSP90 / Heat Shock Protein 90 Monoclonal Antibody | anti-HSP90 antibody
Mouse Monoclonal [clone 4F10] (IgG2b,k) to Human HSP90 / Heat Shock Protein 90
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein G Purified
Synonyms
HSP90 / Heat Shock Protein 90; Monoclonal Antibody; Mouse Monoclonal [clone 4F10] (IgG2b; k) to Human HSP90 / Heat Shock Protein 90; Anti-HSP90 / Heat Shock Protein 90 Antibody (aa1-732; clone 4F10) IHC-plus; Heat Shock Protein 90; Human HSP90; anti-HSP90 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
4F10
Purity/Purification
Protein G Purified
Form/Format
PBS, pH 7.4, 0.1% sodium azide
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-HSP90 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (5 ug/ml), WB (1:1000 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
Recombinant human Hsp90 (1-732aa) purified from E Coli.
Immunogen Type
Recombinant protein
Immunogen
Recombinant human Hsp90 (1-732aa) purified from E Coli.
Antigen Modification
aa1-732
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Cell lysates of HeLa (each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp90(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.)
Western Blot (WB)
(Cell lysates of HeLa (each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp90(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.)
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Product Notes
The HSP90 (Catalog #AAA245471) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 4F10] (IgG2b,k) to Human HSP90 / Heat Shock Protein 90 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSP90 / Heat Shock Protein 90 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (5 ug/ml), WB (1:1000 - 1:2000) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the HSP90 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP90 / Heat Shock Protein 90, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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