Mouse anti-Human NPM1 / NPM / Nucleophosmin Monoclonal Antibody | anti-NPM1 antibody

Mouse Monoclonal [clone 5E3] (IgG2b,k) to Human NPM1 / NPM / Nucleophosmin

Cat. Num. AAA245427

• Gene Names: NPM1; B23; NPM
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein G Purified
• Synonyms: NPM1 / NPM / Nucleophosmin; Monoclonal Antibody; Mouse Monoclonal [clone 5E3] (IgG2b; k) to Human NPM1 / NPM / Nucleophosmin; Anti-NPM1 / NPM / Nucleophosmin Antibody (aa81-294; clone 5E3) IHC-plus; NPM1; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; B23; Nucleophosmin; Human NPM1; anti-NPM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 5E3
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.4, 0.1% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 294

• Applicable Applications for anti-NPM1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human NPM (81-294aa) purified from E Coli.
• Immunogen Type: Recombinant protein
• Immunogen: NPM1 / NPM / Nucleophosmin antibody was raised against recombinant human NPM (81-294aa) purified from E Coli.
• Antigen Modification: aa81-294
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-NPM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(The HeLa and HepG2 cell lysates (5 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-NPM1 antibody

NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.

NCBI and Uniprot Product Information

• NCBI GI #: 10835063
• NCBI GeneID: 4869
• NCBI Accession #: NP_002511.1
• NCBI GenBank Nucleotide #: NM_002520.6
• UniProt Accession #: P06748; P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6
• Molecular Weight: 28,400 Da
• NCBI Official Full Name: nucleophosmin isoform 1
• NCBI Official Synonym Full Names: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM
• UniProt Entry Name: NPM_HUMAN

NCBI Description

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Translation; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; nucleus; cytosol; ribonucleoprotein complex

Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal large subunit binding; NF-kappaB binding; protein binding; Tat protein binding; protein heterodimerization activity; ribosomal small subunit binding

Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; signal transduction; DNA repair; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; nucleosome assembly; negative regulation of cell proliferation; protein localization; regulation of endodeoxyribonuclease activity; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis