Rabbit anti-Human USP8 Polyclonal Antibody | anti-USP8 antibody

USP8, CT (Ubiquitin Carboxyl-terminal Hydrolase 8, Ubiquitin Thiolesterase 8, Ubiquitin-specific processing Protease 8, Deubiquitinating Enzyme 8, Ubiquitin Isopeptidase Y, hUBPy, KIAA0055, UBPY) (FITC)

Cat. Num. AAA6505073

• Gene Names: USP8; UBPY; PITA4; SPG59; HumORF8
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: USP8; Polyclonal Antibody; CT (Ubiquitin Carboxyl-terminal Hydrolase 8; Ubiquitin Thiolesterase 8; Ubiquitin-specific processing Protease 8; Deubiquitinating Enzyme 8; Ubiquitin Isopeptidase Y; hUBPy; KIAA0055; UBPY) (FITC); anti-USP8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP8.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-USP8 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human USP8 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-USP8 Pab is used in Western blot to detect USP8 in A375 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-USP8 antibody

Antibodies; Ubiquitin

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Soncini, C., et al., Oncogene 20(29):3869-3879 (2001). 3. Nagase, T., et al., DNA Res. 3(1):17-24 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 83405557
• NCBI GeneID: 9101
• UniProt Accession #: P40818; Q2TB31; Q7Z3U2; Q86VA0; Q8IWI7; B4DKA8
• Molecular Weight: 115,080 Da
• NCBI Official Full Name: Ubiquitin specific peptidase 8
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 8
• NCBI Official Symbol: USP8
• NCBI Official Synonym Symbols: UBPY; PITA4; SPG59; HumORF8
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 8
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 8
• UniProt Gene Name: USP8
• UniProt Synonym Gene Names: KIAA0055; UBPY; hUBPy

NCBI Description

This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.