Human Arginyl tRNA Synthetase (RARS) ELISA Kit | RARS elisa kit

Human Arginyl tRNA Synthetase (RARS) ELISA Kit

Cat. Num. AAA453191

• Gene Names: RARS; HLD9; ArgRS; DALRD1
• Reactivity: Human
• Synonyms: Arginyl tRNA Synthetase (RARS); Human Arginyl tRNA Synthetase (RARS) ELISA Kit; ArgRS; DALRD1; Arginine tRNA Ligase 1; Cytoplasmic; RARS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RARS. No significant cross-reactivity or interference between RARS and analogues was observed.
• Sequence Length: 660

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.30ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RARS were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RARS were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RARS elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RARS in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RARS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15149476
• NCBI GeneID: 5917
• NCBI Accession #: NP_002878.2
• NCBI GenBank Nucleotide #: NM_002887.3
• UniProt Accession #: P54136; Q53GY4; Q9BWA1; B2RBS9
• Molecular Weight: 67,140 Da
• NCBI Official Full Name: arginine--tRNA ligase, cytoplasmic
• NCBI Official Synonym Full Names: arginyl-tRNA synthetase
• NCBI Official Symbol: RARS
• NCBI Official Synonym Symbols: HLD9; ArgRS; DALRD1
• NCBI Protein Information: arginine--tRNA ligase, cytoplasmic
• UniProt Protein Name: Arginine--tRNA ligase, cytoplasmic
• Protein Family: Arginine--tRNA ligase
• UniProt Gene Name: RARS
• UniProt Synonym Gene Names: ArgRS

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008]

Uniprot Description

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.