Rabbit PBK Polyclonal Antibody | anti-PBK antibody

PBK (PDZ Binding Kinase, Cancer/testis Antigen 84, CT84, Lymphokine-activated Killer T-cell-originated Protein Kinase, MAPKK-like Protein Kinase, Nori-3, Spermatogenesis-related Protein Kinase, SPK, T-LAK Cell-originated Protein Kinase, TOPK)

Cat. Num. AAA615639

• Gene Names: PBK; SPK; CT84; TOPK; Nori-3; FLJ14385
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: PBK; Polyclonal Antibody; PBK (PDZ Binding Kinase; Cancer/testis Antigen 84; CT84; Lymphokine-activated Killer T-cell-originated Protein Kinase; MAPKK-like Protein Kinase; Nori-3; Spermatogenesis-related Protein Kinase; SPK; T-LAK Cell-originated Protein Kinase; TOPK); Anti -PBK (PDZ Binding Kinase; anti-PBK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total PBK/TOPK protein.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5,, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-PBK antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry: (Paraffin) 1:50; IHC Protocol: Citrate/TBST; Immunocytochemistry: (IF) 1:100
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to amino acids at the carboxy-terminus of human PBK/TOPK.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 and PC12 cells, using PBK/TOPK Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing membrane and cytoplasmic localization, using PBK/TOPK Antibody.)

Testing Data

Related Product Information for anti-PBK antibody

PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-Binding domain, which is thought to interact with the tumor suppressor protein hDlg (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly down regulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclinB has been shown to phosphorylate PBK/TOPK in vitro, presumably at Thr9 (1). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4).

Product Categories/Family for anti-PBK antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 119583941
• NCBI GeneID: 55872
• UniProt Accession #: Q96KB5; Q9NPD9; Q9NYL7; Q9NZK6; D3DST2
• Molecular Weight: 36,085 Da
• NCBI Official Full Name: PDZ binding kinase, isoform CRA_a
• NCBI Official Synonym Full Names: PDZ binding kinase
• NCBI Official Symbol: PBK
• NCBI Official Synonym Symbols: SPK; CT84; TOPK; Nori-3; FLJ14385
• NCBI Protein Information: lymphokine-activated killer T-cell-originated protein kinase; OTTHUMP00000128310; OTTHUMP00000225324; PDZ-binding kinase; cancer/testis antigen 84; MAPKK-like protein kinase; serine/threonine protein kinase; T-LAK cell-originated protein kinase; spermatogenesis-related protein kinase
• UniProt Protein Name: Lymphokine-activated killer T-cell-originated protein kinase
• Protein Family: Lymphokine-activated killer T-cell-originated protein kinase
• UniProt Gene Name: PBK
• UniProt Synonym Gene Names: TOPK
• UniProt Entry Name: TOPK_HUMAN

NCBI Description

This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq]

Uniprot Description

PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.12.2; Cancer Testis Antigen (CTA); Protein kinase, Ser/Thr (non-receptor); Other group; TOPK family

Chromosomal Location of Human Ortholog: 8p21.2

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: mitosis; negative regulation of protein amino acid phosphorylation; negative regulation of inflammatory response; negative regulation of stress-activated MAPK cascade; protein amino acid phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process