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Testing Data (Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PRMT1 (0.1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PRMT1 (42kD).)

Rabbit anti-Human, Mouse PRMT1 Polyclonal Antibody | anti-PRMT1 antibody

PRMT1 (Protein arginine N Methyltransferase 1, ANM1, HCP1, HRMT1L2, IR1B4) (MaxLight 490)

Gene Names
PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
Reactivity
Human, Mouse
Applications
Immunoblot, Western Blot, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PRMT1; Polyclonal Antibody; PRMT1 (Protein arginine N Methyltransferase 1; ANM1; HCP1; HRMT1L2; IR1B4) (MaxLight 490); anti-PRMT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognize the PRMT1 doublet, Mr ~42-44kD. A non-specific protein was also detected, Mr ~120kD. Species Crossreactivity: Human and mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Sequence Length
1450
Applicable Applications for anti-PRMT1 antibody
Immunoblot (IB), Western Blot (WB)
Application Notes
IB: 0.1-0.5ug/ml of this lot detected PRMT1 in RIPA lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells.
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated, synthetic peptide corresponding to aa298-318 (KTGEEIFGTIGMRPNAKNNRD) of human protein arginine methyltransferase 1 (accession number CAA71765). The immunizing sequence is identical in mouse.
Conjugate
MaxLight490
Included Positive Antigen Control
Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PRMT1 (0.1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PRMT1 (42kD).)

Testing Data (Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PRMT1 (0.1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PRMT1 (42kD).)
Product Categories/Family for anti-PRMT1 antibody
References
1. Cote J., et. al., Mol. Bio. Cell 14: 274-287 (2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
4244
NCBI Official Full Name
Homo sapiens protein arginine methyltransferase 1 (PRMT1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
protein arginine methyltransferase 1
NCBI Official Symbol
PRMT1
NCBI Official Synonym Symbols
ANM1; HCP1; IR1B4; HRMT1L2
NCBI Protein Information
protein arginine N-methyltransferase 1
UniProt Protein Name
Protein arginine N-methyltransferase 1
UniProt Gene Name
PRMT1
UniProt Synonym Gene Names
HMT2; HRMT1L2; IR1B4
UniProt Entry Name
ANM1_HUMAN

NCBI Description

This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.125; Nuclear receptor co-regulator; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein-arginine omega-N asymmetric methyltransferase activity; histone methyltransferase activity; methyltransferase activity; protein binding; N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation; establishment and/or maintenance of chromatin architecture; cell surface receptor linked signal transduction; protein amino acid methylation; regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of megakaryocyte differentiation; neurite development

Research Articles on PRMT1

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Product Notes

The PRMT1 prmt1 (Catalog #AAA6494331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRMT1 (Protein arginine N Methyltransferase 1, ANM1, HCP1, HRMT1L2, IR1B4) (MaxLight 490) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT1 can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), Western Blot (WB). IB: 0.1-0.5ug/ml of this lot detected PRMT1 in RIPA lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRMT1 prmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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