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Western Blot (WB) (HSP40 Antibody western blot analysis in Hela,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the HSP40 antibody detected the HSP40 protein (arrow).)

Rabbit anti-Human HSP40 Polyclonal Antibody | anti-DNAJB1 antibody

HSP40 Antibody

Gene Names
DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HSP40; Polyclonal Antibody; HSP40 Antibody; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; Heat shock protein 40; Human DnaJ protein 1; hDj-1; DNAJB1; DNAJ1; HDJ1; HSPF1; anti-DNAJB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HSP40 antibody is generated from rabbits immunized with a recombinant protein encoding full length of human HSP40.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Length
240
Applicable Applications for anti-DNAJB1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Recombinant Protein
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(HSP40 Antibody western blot analysis in Hela,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the HSP40 antibody detected the HSP40 protein (arrow).)

Western Blot (WB) (HSP40 Antibody western blot analysis in Hela,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the HSP40 antibody detected the HSP40 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSP40 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSP40 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-DNAJB1 antibody
DnaJ (Hsp40) belongs to the DnaJ-class of molecular chaperones with a C-terminal Zn finger domain. HSP40 (DnaJ) together with DnaK and GrpE form a molecular chaperone that is involved in formation of protein complexes, protein folding, prevention of protein aggregation, and protein turnover and export. Several human neurodegenerative diseases involve the expansion of a polyglutamine within the disease proteins. Molecular chaperones such as HSP40 complexes can modulate polyglutamine pathogenesis In transgenic Drosophila disease models of Machado-Joseph disease and Huntington disease Hdj1, the Drosophila homolog to human HSP40, demonstrates substrate specificity for polyglutamine proteins suppression in combination with other molecular chapterones of neurotoxicity, and altered solubility of mutant polyglutamine proteins.
Product Categories/Family for anti-DNAJB1 antibody
References
Ohtsuka, K., et al., Cell Stress Chaperones 5(2):98-112 (2000). Hata, M., et al., Biochim. Biophys. Acta 1397(1):43-55 (1998). Hata, M., et al., Genomics 38(3):446-449 (1996). Ohtsuka, K., Biochem. Biophys. Res. Commun. 197(1):235-240 (1993).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38044
NCBI Official Full Name
dnaJ homolog subfamily B member 1 isoform 1
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily B, member 1
NCBI Official Symbol
DNAJB1
NCBI Official Synonym Symbols
Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
NCBI Protein Information
dnaJ homolog subfamily B member 1
UniProt Protein Name
DnaJ homolog subfamily B member 1
Protein Family
UniProt Gene Name
DNAJB1
UniProt Synonym Gene Names
DNAJ1; HDJ1; HSPF1; HSP40; Heat shock protein 40; hDj-1
UniProt Entry Name
DNJB1_HUMAN

NCBI Description

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.

Research Articles on DNAJB1

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Product Notes

The DNAJB1 dnajb1 (Catalog #AAA9208354) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP40 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSP40 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the DNAJB1 dnajb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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