Rat HSF2 Monoclonal Antibody | anti-HSF2 antibody

HSF2 Antibody

Cat. Num. AAA8001878

• Gene Names: HSF2; HSF 2; HSTF 2
• Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit, Dog, Bovine, Sheep, Pig
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein G Purified
• Synonyms: HSF2; Monoclonal Antibody; HSF2 Antibody; Rat Anti-Mouse HSF2 Monoclonal IgG; Clone 3E2; HSTF2 Antibody; Heat shock factor protein 2 Antibody; Heat shock transcription factor 2 Antibody; HSF 2 Antibody; anti-HSF2 antibody

• Host: Rat
• Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit, Dog, Bovine, Sheep, Pig
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 3E2
• Specificity: Detects ~69kDa.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 518

• Applicable Applications for anti-HSF2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Microarray (AM)
• Application Notes: WB: 1:250; ICC/IF: 1:200
• Immunogen: Purified recombinant mouse HSF2 protein
• Immunogen Species: Mouse
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm, Nucleus
• Certificate of Analysis: 4 ug/ml of SMC-119 was sufficient for detection of HSF2 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 . Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Western Blot (WB)

(Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 . Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 . Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Related Product Information for anti-HSF2 antibody

HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Product Categories/Family for anti-HSF2 antibody

Cancer; Heat Shock; Cell Signaling; Epigenetics and Nuclear Signaling

References

1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934. 2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82. 3.Tanaka KI et al. (2007). JBC Papers Online Manuscript M704081200. 4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796. 5. McMillan D. R., Xiao X., Shao L., Graves K., and Benjamin I. J. (1998) J Bio Chem 273: 7523-7528. 6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769-6774. 7. Fiorenza M.T., Farkas T., Dissing M., Kolding D. and Zimarino V. (1995) Nucleic Acids Res. 23 (3):467-474. 8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293. 9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397. 10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334-1343. 11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.

NCBI and Uniprot Product Information

• NCBI GI #: 207113147
• NCBI GeneID: 3298
• NCBI Accession #: NP_001129036.1
• NCBI GenBank Nucleotide #: NM_001135564.1
• UniProt Accession #: P38533 ; Q03933
• NCBI Official Full Name: heat shock factor protein 2 isoform b
• NCBI Official Synonym Full Names: heat shock transcription factor 2
• NCBI Official Symbol: HSF2
• NCBI Official Synonym Symbols: HSF 2; HSTF 2
• NCBI Protein Information: heat shock factor protein 2
• Protein Family: Heat shock factor protein

NCBI Description

The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]