Western Blot (WB)
(Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.)
Rat HSF2 Monoclonal Antibody | anti-HSF2 antibody
HSF2 Antibody: PerCP
Western Blot (WB)
(Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.)
Western Blot (WB)
(Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.)
Scientific Background: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Uniprot Description
HSF2: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Cellular Component: cytoplasm; nucleus
Molecular Function: protein homodimerization activity; DNA binding; sequence-specific DNA binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis
Research Articles on HSF2
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Product Notes
The HSF2 hsf2 (Catalog #AAA802375) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The HSF2 Antibody: PerCP reacts with Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit, Dog, Bovine, Sheep, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's HSF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). 4ug/ml was sufficient for detection of HSF2 of HeLa lysate. Researchers should empirically determine the suitability of the HSF2 hsf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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