Rabbit anti-Human HSF1  Monoclonal Antibody | anti-HSF1  antibody

Rabbit anti Human HSF1  Monoclonal Antibody

Cat. Num. AAA2544353

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Synonyms: HSF1 ; Monoclonal Antibody; Rabbit anti Human HSF1 Monoclonal Antibody; anti-HSF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 833

• Applicable Applications for anti-HSF1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human HSF1. The detection limit for Human HSF1 is approximately 0.00975 ng/well.; IHC-P: 1-10 mug/mL
• Immunogen: Recombinant Human HSF1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-HSF1  antibody

Heat shock factor protein 1, also known as heat shock transcription factor 1, HSF1 and HSTF1, is a cytoplasm and nucleus protein which belongs to the HSF family. HSF1 is the major transcription factor of HSPs (heat shock proteins) in response to various stresses. Wild type HSF1 (heat shock transcriptional factor 1) is normally inactive. HSF1/HSTF1 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF1/HSTF1 protects cells and organisms against various types of stress, either by triggering a complex response that promotes cell survival or by triggering cell death when stress-induced alterations cannot be rescued. HSF1/HSTF1 is the key protein in regulating stress response. It can be activated under heat, oxidative or another stress conditions. Dominant-positive and dominant-negative HSF1 are two types of HSF1 mutants. Both of them gain the DNA binding activity in the absence of stress. In addition, dominant-positive HSF1 acquires transcriptional activity, which dominant-negative HSF1 does not acquire. HSF1/HSTF1 was also reported to contribute to cell resistance against genotoxic stress, such as that caused by doxorubicin, an anticancer drug in common clinical use.

NCBI and Uniprot Product Information

• NCBI GI #: 1322586
• UniProt Accession #: P10961; P11529; D6VU70
• Molecular Weight: 93,282 Da
• NCBI Official Full Name: HSF1
• UniProt Protein Name: Heat shock factor protein
• UniProt Gene Name: HSF1
• UniProt Synonym Gene Names: HSF; HSTF
• UniProt Entry Name: HSF_YEAST

Uniprot Description

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures.