Rabbit HSF1 Polyclonal Antibody | anti-HSF1 antibody

HSF1 Antibody (Ser303)

Cat. Num. AAA5313679

• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Chromatography
• Synonyms: HSF1; Polyclonal Antibody; HSF1 Antibody (Ser303); Rabbit Polyclonal HSF1 Antibody (Ser303); Polyclonal HSF1 antibody; Anti-HSF1 antibody; Heat shock factor protein 1 antibody; Heat shock transcription factor 1 antibody; HSF 1 antibody; HSF1 antibody; HSTF 1 antibody; HSTF1 antibody; anti-HSF1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HSF1 antibody (Ser303) detects endogenous levels of HSF1 only when phosphorylated at serine 303
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 833

• Applicable Applications for anti-HSF1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100
• Immunogen: HSF1 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 303 (P-P-SP-P-P) as the immunogen.
• Preparation and Storage: Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 antibody (Ser303))

Western Blot (WB)

(Western blot analysis of extracts from MCF7 cells, using HSF1 (phospho-Ser303) antibody)

Related Product Information for anti-HSF1 antibody

HSF1 is a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed.

Product Categories/Family for anti-HSF1 antibody

Protein Modification & Stress Response

NCBI and Uniprot Product Information

• NCBI GI #: 1322586
• NCBI Official Full Name: HSF1
• Protein Family: Heat shock factor protein