Rabbit anti-Mouse, Human Epha4 Polyclonal Antibody | anti-Epha4 antibody

Epha4, ID (Epha4, Sek, Sek1, Ephrin type-A receptor 4, Tyrosine-protein kinase receptor MPK-3, Tyrosine-protein kinase receptor SEK-1) (Azide free) (HRP)

Cat. Num. AAA6296000

• Gene Names: Epha4; rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Epha4; Polyclonal Antibody; ID (Epha4; Sek; Sek1; Ephrin type-A receptor 4; Tyrosine-protein kinase receptor MPK-3; Tyrosine-protein kinase receptor SEK-1) (Azide free) (HRP); Tyrosine-protein kinase receptor SEK-1; anti-Epha4 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Epha. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Epha4 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 464-492 in the Central region of mouse Epha.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of K562 cell line lysates (35ug/lane) usingMBS6009595.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue using MBS6009595 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-Epha4 antibody

Antibodies; Protein Kinases

References

1. Kuwako, K., et al. Neuron 67(3):407-421(2010). 2. Qu, Y., et al. J. Neurosci. 30(28):9392-9401(2010). 3. Oginuma, M., et al. Development 137(9):1515-1522(2010). 4. Xie, Z., et al. Proc. Natl. Acad. Sci. U.S.A. 107(14):6510-6515(2010). 5. Galimberti, I., et al. Neuron 65(5):627-642(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 34328113
• NCBI GeneID: 13838
• NCBI Accession #: NP_031962.2
• NCBI GenBank Nucleotide #: NM_007936.3
• UniProt Accession #: Q03137; Q80VZ2
• Molecular Weight: 103,984 Da
• NCBI Official Full Name: ephrin type-A receptor 4
• NCBI Official Synonym Full Names: Eph receptor A4
• NCBI Official Symbol: Epha4
• NCBI Official Synonym Symbols: rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
• NCBI Protein Information: ephrin type-A receptor 4
• UniProt Protein Name: Ephrin type-A receptor 4
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha4
• UniProt Synonym Gene Names: Sek; Sek1

Uniprot Description

EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 1 C4|1 39.55 cM

Cellular Component: axon; cell projection; cell surface; cytoplasm; dendrite; dendritic spine; early endosome membrane; endoplasmic reticulum; filopodium; Golgi apparatus; integral to plasma membrane; mitochondrial outer membrane; nerve terminal; neuromuscular junction; perikaryon; plasma membrane; postsynaptic density

Molecular Function: ephrin receptor binding; GPI-linked ephrin receptor activity; PH domain binding; protein binding; protein kinase activity; transmembrane-ephrin receptor activity

Biological Process: adult walking behavior; axon guidance; corticospinal tract morphogenesis; glial cell migration; motor axon guidance; negative regulation of axon regeneration; peptidyl-tyrosine phosphorylation; positive regulation of dendrite morphogenesis; positive regulation of JNK activity; protein amino acid autophosphorylation; regulation of astrocyte differentiation; regulation of axonogenesis; regulation of GTPase activity