Western Blot (WB)
(Western blot analysis of extracts of various cells, using WRN antibody.)
Rabbit anti-Human WRN Polyclonal Antibody | anti-WRN antibody
WRN Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cells, using WRN antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cells, using WRN antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]
Uniprot Description
WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.
Protein type: Cell cycle regulation; DNA repair, damage; DNA-binding; EC 3.6.4.12; Helicase; Nucleolus
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: centrosome; cytoplasm; MutLalpha complex; nuclear chromosome, telomeric region; nucleolus; nucleoplasm
Molecular Function: 3'-5' DNA helicase activity; 3'-5' exonuclease activity; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATPase activity; bubble DNA binding; DNA binding; DNA helicase activity; exonuclease activity; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; magnesium ion binding; manganese ion binding; protein binding; protein complex binding; protein homodimerization activity; Y-form DNA binding
Biological Process: base-excision repair; cell aging; cellular response to starvation; DNA metabolic process; DNA replication; DNA synthesis during DNA repair; double-strand break repair; multicellular organismal aging; nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; protein sumoylation; regulation of apoptosis; replication fork processing; response to DNA damage stimulus; response to oxidative stress; response to UV-C; strand displacement; telomere maintenance; telomere maintenance via recombination
Disease: Werner Syndrome
Research Articles on WRN
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Product Notes
The WRN wrn (Catalog #AAA2540812) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WRN Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WRN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200 - 1:1000. Researchers should empirically determine the suitability of the WRN wrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WRN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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