Mouse Hexokinase-3 Monoclonal Antibody | anti-HK3 antibody

Mouse Anti Human Hexokinase-3

Cat. Num. AAA140266

• Gene Names: HK3; HXK3; HKIII
• Applications: ELISA, Western Blot
• Purity: Hexokinase-3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Hexokinase-3; Monoclonal Antibody; Mouse Anti Human Hexokinase-3; HK3 Antibody; Mouse Anti Human; EC 2.7.1.1; Hexokinase type III; HK III; HXK3; HK3; anti-HK3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: P4F5AT
• Purity/Purification: Hexokinase-3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 923

• Applicable Applications for anti-HK3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Hexokinase-3 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
• Immunogen: Anti-human Hexokinase-3 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Hexokinase-3 amino acids 1-923 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-HK3 antibody

Introduction: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK3 encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. Hexokinase3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase3 may have anabolic functions, providing H6P for glycogen or lipid synthesis.

Product Categories/Family for anti-HK3 antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 194097330
• NCBI GeneID: 3101
• NCBI Accession #: NP_002106.2
• NCBI GenBank Nucleotide #: NM_002115.2
• Molecular Weight: 99,025 Da
• NCBI Official Full Name: hexokinase-3
• NCBI Official Synonym Full Names: hexokinase 3 (white cell)
• NCBI Official Symbol: HK3
• NCBI Official Synonym Symbols: HXK3; HKIII
• NCBI Protein Information: hexokinase-3; ATP:D-hexose 6-phosphotransferase; HK III; hexokinase type III
• UniProt Protein Name: Hexokinase-3
• Protein Family: Hexokinase
• UniProt Gene Name: HK3
• UniProt Synonym Gene Names: HK III
• UniProt Entry Name: HXK3_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]

Uniprot Description

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - starch and sucrose; Kinase, other; Carbohydrate Metabolism - galactose; EC 2.7.1.1; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Enzyme, cellular metabolism; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transferase

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: protein complex; mitochondrion; cytosol

Molecular Function: hexokinase activity; enzyme binding; mannokinase activity; hormone binding; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; carbohydrate metabolic process; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport