Hexokinase Type III Recombinant Protein | HK3 recombinant protein

Hexokinase Type III, Recombinant, Human (HK3, HKIII)

Cat. Num. AAA635722

• Gene Names: HK3; HXK3; HKIII
• Purity: Highly Purified; 95% by SDS PAGE
• Synonyms: Hexokinase Type III; Recombinant; Human (HK3; HKIII); HK3 recombinant protein

• Host: Human
• Purity/Purification: Highly Purified; 95% by SDS PAGE
• Form/Format: Supplied as a liquid in 20mM Tris-HCl, pH8.0, 10% glycerol

• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(15% SDS PAGE)

Related Product Information for HK3 recombinant protein

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase3 may have anabolic functions, providing H6P for glycogen or lipid synthesis. Recombinant human Hexokinase3, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Categories/Family for HK3 recombinant protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 194097330
• NCBI GeneID: 3101
• NCBI Accession #: NP_002106.2
• NCBI GenBank Nucleotide #: NM_002115.2
• UniProt Accession #: P52790; Q8N1E7
• Molecular Weight: 101.1 kD
• NCBI Official Full Name: hexokinase-3
• NCBI Official Synonym Full Names: hexokinase 3 (white cell)
• NCBI Official Symbol: HK3
• NCBI Official Synonym Symbols: HXK3; HKIII
• NCBI Protein Information: hexokinase-3; HK III; hexokinase type III; ATP:D-hexose 6-phosphotransferase
• UniProt Protein Name: Hexokinase-3
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK3
• UniProt Synonym Gene Names: HK III
• UniProt Entry Name: HXK3_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]

Uniprot Description

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transferase; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.7.1.1; Kinase, other; Enzyme, cellular metabolism; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: protein complex; mitochondrion; cytosol

Molecular Function: hexokinase activity; enzyme binding; mannokinase activity; hormone binding; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; carbohydrate metabolic process; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport