Mouse anti-Human, Mouse Heterochromatin Protein 1, beta Monoclonal Antibody | anti-Su(var)205 antibody

Heterochromatin Protein 1, beta (Heterochromatin Protein 1 beta (HP1-beta, BX1, Chromobox homolog 1, HP1Hs-beta, MOD1, Modifier 1 protein, Heterochromatin protein p25, M31)

Cat. Num. AAA606710

• Gene Names: Su(var)205; CBX5; CG8409; dHP-1a; dHP1; DmelCG8409; DmHP-1; DmHP1; E(var)29; E(var)29A; FBgn0003607; H3; HP-1; hp1; Hp1; HP1; HP1-VS; hp1a; Hp1a; HP1a; HP1A; HP1alpha; Su(var); Su(var)2-05; Su(var)2-5; Su(Var)2-5; Su(var)2-501; su(var)205; SU(VAR)205; Su(var)29A; Su(
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: Heterochromatin Protein 1; beta; Monoclonal Antibody; beta (Heterochromatin Protein 1 beta (HP1-beta; BX1; Chromobox homolog 1; HP1Hs-beta; MOD1; Modifier 1 protein; Heterochromatin protein p25; M31); Anti -Heterochromatin Protein 1; anti-Su(var)205 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 2Q2289
• Specificity: Recognizes mouse Heterochromatin protein-1 beta (HP1b). Species Crossreactivity: human.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in 40% glycerol. No preservative added.

• Applicable Applications for anti-Su(var)205 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Immunofluorescence: 1:250-1:2500; ELISA: 1:250-1:2500; Western Blot: 1:250-1:2500; Immunohistochemistry: 1:250-1:2500; Immunoprecipitation: 1:250-1:2500
• Immunogen: Recombinant mouse HP1b.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Confocal fluorescent analysis of HeLa, A431 and NIH/3T3 cells using MBS606710 (Red). Actin filaments have been labeled with AlexaFluor® 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining)

Testing Data

(NIH/3T3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with HP-1 beta (1:500 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein HP-1, beta (~28kD))

Testing Data

(Jurkat lysate (Lane 1), L6 lysate (Lane 2), Mouse brain lysate (Lane 3), NIH/3T3 lysate (Lane 4), PC12 lysate (Lane 5) and PC3 lysate (Lane 6) were resolved by electrophoresis, transferred to PVDF membrane and probed with HP1 (1:500 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein HP-1, beta (~28kD))

Product Categories/Family for anti-Su(var)205 antibody

Antibodies; Abs to Chromatin Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1170337
• NCBI GeneID: 34119
• UniProt Accession #: P05205; Q9VLR6; A4V0F1; B6UVR4; B6UVS1; B6UVS2; B6UVS3; B6UVT2; B6UVT6; B6UVT7
• Molecular Weight: 23,185 Da
• NCBI Official Full Name: Heterochromatin protein 1
• NCBI Official Synonym Full Names: Suppressor of variegation 205
• NCBI Official Symbol: Su(var)205
• NCBI Official Synonym Symbols: CBX5; CG8409; dHP-1a; dHP1; DmelCG8409; DmHP-1; DmHP1; E(var)29; E(var)29A; FBgn0003607; H3; HP-1; hp1; Hp1; HP1; HP1-VS; hp1a; Hp1a; HP1a; HP1A; HP1alpha; Su(var); Su(var)2-05; Su(var)2-5; Su(Var)2-5; Su(var)2-501; su(var)205; SU(VAR)205; Su(var)29A; Su(
• NCBI Protein Information: CG8409 gene product from transcript CG8409-RA; C1A9-nuclear-antigen; CG8409-PA; CG8409-PB; Su(var)2-5; Su(var)205-PA; Su(var)205-PB; heterochromatic protein; heterochromatic protein 1a; heterochromatin protein 1; heterochromatin protein 1A; heterochromatin protein-1; heterochromatin-binding protein; heterochromatin-protein-1; histone protein 1; suppressor of variegation 205
• UniProt Protein Name: Heterochromatin protein 1
• UniProt Gene Name: Su(var)205
• UniProt Synonym Gene Names: HP1; HP1
• UniProt Entry Name: HP1_DROME

Uniprot Description

Function: Structural component of heterochromatin, involved in gene repression and the modification of position-effect-variegation. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression.

Subunit structure: Homodimer; probably associates with Su(var)3-9. Interacts with Mcm10. Interacts (via chromoshadow domain) with piwi (via N-terminal region). Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location: Nucleus.

Sequence similarities: Contains 2 chromo domains.