Mouse anti-Human Heparin Binding EGF-like Growth Factor Monoclonal Antibody | anti-HBEGF antibody

Heparin Binding EGF-like Growth Factor (HB-EGF, HBEGF, Diphtheria Toxin Receptor, DT-R, DTR)

Cat. Num. AAA603983

• Gene Names: HBEGF; DTR; DTS; DTSF; HEGFL
• Reactivity: Human
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Heparin Binding EGF-like Growth Factor; Monoclonal Antibody; Heparin Binding EGF-like Growth Factor (HB-EGF; HBEGF; Diphtheria Toxin Receptor; DT-R; DTR); Anti -Heparin Binding EGF-like Growth Factor (HB-EGF; anti-HBEGF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7H229
• Specificity: Recognizes human Heparin Binding EGF-like Growth Factor (HB-EGF). Does not crossreact with recombinant human Amphiregulin, BTC, HRG-alpha, HRG-beta, EGF, TGF-alpha or recombinant mouse Epigen or Epiregulin.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
• Concentration: ~0.5 mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for anti-HBEGF antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in Western Blot, Neutralization and ELISA.; Dilution: Western Blot: 1ug/ml using recombinant human Heparin Binding EGF-like Growth Factor.; Neutralization: ~0.5-2.5ug/ml yields one-half maximal inhibition (ND50) of the biological activity of recombinant human HB-EGF (5ng/ml). Measured by its ability to neutralize HB-EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line.
• Immunogen: Recombinant protein corresponding to aa63-148 of human HB-EGF expressed in Sf21. Species Sequence Homology (77%): rat and mouse
• Endotoxin Level: <0.1EU/ug (LAL).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HBEGF antibody

Heparin-binding EGF-like Growth Factor (HB-EGF) is an EGF family growth factor that is synthesized as a transmembrane precursor (proHB-EGF). ProHB-EGF, which contains one EGF-like domain, is subject to variable O-linked glycosylation and N-terminal truncation. In human, this precursor is the cellular receptor for diphtheria toxin. ADAM12 or ADAM17-mediated cleavage of proHB-EGF releases a 22 kD soluble HB-EGF; the cytoplasmic fragment functions as a transcriptional repressor. HB-EGF directly activates EGF R and ErbB4 and promotes the development of many tissues. Human HB-EGF shares 77% aa sequence identity with mouse and rat HB-EGF.

Product Categories/Family for anti-HBEGF antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 183867
• NCBI GeneID: 1839
• UniProt Accession #: Q99075; B2R821; Q14487; Q53H93; Q9UMJ6
• Molecular Weight: 23,067 Da
• NCBI Official Full Name: heparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin-binding EGF-like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: DTR; DTS; DTSF; HEGFL
• NCBI Protein Information: proheparin-binding EGF-like growth factor; OTTHUMP00000159876; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• Protein Family: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: DTR; DTS; HEGFL
• UniProt Entry Name: HBEGF_HUMAN

Uniprot Description

HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; wound healing, spreading of epidermal cells; positive regulation of cell migration