Mouse Hemagglutinin Tag Monoclonal Antibody
Hemagglutinin Tag (YPYDVPDYA) (HA Tag)
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Hemagglutinin Tag; Monoclonal Antibody; Hemagglutinin Tag (YPYDVPDYA) (HA Tag); Anti -Hemagglutinin Tag (YPYDVPDYA) (HA Tag); anti-Hemagglutinin Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3G165
Specificity
Recognizes HA tagged fusion proteins. Species Crossreactivity: broad.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Applicable Applications for anti-Hemagglutinin Tag antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunogen
Synthetic peptide containing the sequence YPYDVPDYA derived from the influenza virus hemaglutinin (HA) protein.
Positive Control
HA-tagged JNK-1 transfected cell lysates
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Hemagglutinin Tag antibody
The HA tag sequence is derived from influenza hemagglutinin and is typically used to facilitate the detection of proteins. Tags such as HA used in conjunction with proteins can aid in purification, identification, and functional analysis of the tagged protein.Through the use of standard molecular biology techniques, a cDNA, which encodes the protein of interest can be cloned, in- frame, into an expression vector containing the tag sequence. The resultant tagged protein is usually referred to as an epitope-tagged protein or a fusion protein. Currently, a variety of expression vectors containing tags are commercially available. Depending on the expression vector selected, these fusion proteins may be expressed in a variety of organisms including bacteria, yeast, insect cells, and mammalian cells. Detection of a tagged protein is facilitated by the use of an antibody directed against the particular tag. This alleviates the need to generate a specific antibody against the protein itself. Therefore, newly identified proteins can be expressed and studied without having to wait for a specific antibody to be generated. Some of the more commonly used fusion tags include: c-myc, DYKDDDDK, GFP, GST, HA, His, and MBP.
Product Categories/Family for anti-Hemagglutinin Tag antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
hemagglutinin
Similar Products
Product Notes
The Hemagglutinin Tag (Catalog #AAA600765) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hemagglutinin Tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml. Researchers should empirically determine the suitability of the Hemagglutinin Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hemagglutinin Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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