Western Blot (WB)
Mouse anti-Human PDL-1 Monoclonal Antibody | anti-PDL-1 antibody
Mouse monoclonal antibody to human PDL-1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Protein G affinity purified
Synonyms
PDL-1; Monoclonal Antibody; Mouse monoclonal antibody to human PDL-1; mAb anti Human PDL-1; clone 10B12; Mouse monoclonal antibody (mAb) to human programmed death ligand-1 (PDL-1); mouse mAb to B7H1; mouse mAb to human CD274; anti-PDL-1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
10B12
Specificity
Reactive with human programmed death ligand-1 (PDL-1).
Purity/Purification
Protein G affinity purified
Form/Format
Lyophilized from a solution in 0.01M PBS, pH 7.0
Applicable Applications for anti-PDL-1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: Indirect ELISA: The antibody reacts with recombinant PDL-1 in indirect ELISA.
Western Blot: Anti-PDL-1 clone 10B12 detected 5ng recombinant PDL-1 on Western Blot. The blot was stained with HRP substrate 4-Chloro-1-Naphthol.
Western Blot: Anti-PDL-1 clone 10B12 detected 5ng recombinant PDL-1 on Western Blot. The blot was stained with HRP substrate 4-Chloro-1-Naphthol.
Target Protein
Human programmed death ligand-1 (PDL-1), also known as B7H1 or CD274.
Immunogen
Human cell expressed recombinant PDL-1 (Phe19-Arg238) with poly-histidine tag at C-terminus.
Fusion Myeloma
SP2/0
Reconstitution
Double distilled water is recommended to reconstitute the antibody to 1mg/mL.
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-PDL-1 antibody
Programmed Death Ligand-1 (PDL-1), a 40KD transmembrane protein, is a ligand of immune checkpoint protein Programmed Death-1 (PD-1). During T-cell receptor signalling, the binding of PDL-1 to PD-1 transmits an inhibitory signal to activated T-cells to decrease their proliferation. PDL-1 is also believed to play a role in the generation and regulation of Treg cells, and in the enhancement of Treg cell function. PDL-1/PD-1 pathway is important to immune suppression, such as self-tolerance, suppression of immune-rejection during pregnancy and allograft, and immune evasion by cancers. Upregulation of PD-L1 has been found to be associated with many cancer types. For example, increased PDL-1 expression has been shown to be related to tumor aggressiveness and higher mortality in patients with renal cell carcinoma, and related to significantly poorer prognosis and lower intraepithelial CD8+ T-lymphocyte count in patience with ovarian cancer. Antibodies that block the PDL-1 /PD-1 pathway have been extensively studied in clinical trials, and some have been approved as immunotherapy for cancer.
Product Categories/Family for anti-PDL-1 antibody
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Product Notes
The PDL-1 (Catalog #AAA592441) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody to human PDL-1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDL-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: Indirect ELISA: The antibody reacts with recombinant PDL-1 in indirect ELISA. Western Blot: Anti-PDL-1 clone 10B12 detected 5ng recombinant PDL-1 on Western Blot. The blot was stained with HRP substrate 4-Chloro-1-Naphthol. Researchers should empirically determine the suitability of the PDL-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDL-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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