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Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using FARSB Antibody. The lane on the left was treated with blocking peptide.)

Rabbit FARSB Polyclonal Antibody | anti-FARSB antibody

FARSB Antibody

Gene Names
FARSB; FRSB; PheHB; PheRS; FARSLB; RILDBC; HSPC173; NEDBLLA
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Sheep, Rabbit
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
FARSB; Polyclonal Antibody; FARSB Antibody; C76708; Farsa; Farsl; Farslb; FRSB; HSPC173; PheHB; Phenylalanine tRNA ligase beta subunit; phenylalanine tRNA ligase 1; beta; cytoplasmic; Phenylalanine tRNA ligase beta chain; Phenylalanine--tRNA ligase beta chain; phenylalanine-tRNA synthetase-like; beta subunit; Phenylalanyl tRNA synthetase beta chain; Phenylalanyl-tRNA synthetase beta chain; phenylalanyl-tRNA synthetase beta-subunit; phenylalanyl-tRNA synthetase-like; PheRS; SYFB_HUMAN; anti-FARSB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Sheep, Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
FARSB Antibody detects endogenous levels of FARSB.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
589
Applicable Applications for anti-FARSB antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human FARSB.
Fragment
Fab Fragment
Conjugate
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using FARSB Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using FARSB Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Colo205, using FARSB Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from Colo205, using FARSB Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-FARSB antibody
Description: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Subunit Structure: Heterotetramer; dimer of two heterodimers formed by FARSA and FARSB.

Similarity: Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 66 kDa
Predicted: 67 kDa
NCBI Official Full Name
Phenylalanine--tRNA ligase beta subunit
NCBI Official Synonym Full Names
phenylalanyl-tRNA synthetase subunit beta
NCBI Official Symbol
FARSB
NCBI Official Synonym Symbols
FRSB; PheHB; PheRS; FARSLB; RILDBC; HSPC173; NEDBLLA
NCBI Protein Information
phenylalanine--tRNA ligase beta subunit
UniProt Protein Name
Phenylalanine--tRNA ligase beta subunit
UniProt Gene Name
FARSB
UniProt Synonym Gene Names
FARSLB; FRSB; PheRS
UniProt Entry Name
SYFB_HUMAN

NCBI Description

This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

FARSLB: a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. [provided by RefSeq, Jul 2008]

Protein type: Ligase; EC 6.1.1.20; Translation

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: RNA binding; phenylalanine-tRNA ligase activity; magnesium ion binding; ATP binding

Biological Process: tRNA aminoacylation for protein translation; translation; phenylalanyl-tRNA aminoacylation; gene expression

Research Articles on FARSB

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Product Notes

The FARSB farsb (Catalog #AAA9611753) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FARSB Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Sheep, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's FARSB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the FARSB farsb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FARSB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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