Mouse HCN4 Monoclonal Antibody | anti-Hcn4 antibody

HCN4 Antibody: FITC

Cat. Num. AAA801089

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
• Synonyms: HCN4; Monoclonal Antibody; HCN4 Antibody: FITC; Hyperpolarization activated cyclic nucleotide gated potassium channel 4; HCN 4; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4; anti-Hcn4 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S114-10
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1198

• Applicable Applications for anti-Hcn4 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: FITC
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Western Blot (WB)

(Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone S114-10. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

Related Product Information for anti-Hcn4 antibody

Background Info: Detects ~130kDa. No cross-reactivity against other HCNs

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3).

Product Categories/Family for anti-Hcn4 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232

NCBI and Uniprot Product Information

• NCBI GI #: 25742571
• NCBI GeneID: 59266
• NCBI Accession #: NP_067690.1
• NCBI GenBank Nucleotide #: NM_021658.1
• UniProt Accession #: Q9JKA7; Q9QZW4
• Molecular Weight: 128,761 Da
• NCBI Official Full Name: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• NCBI Official Synonym Full Names: hyperpolarization activated cyclic nucleotide-gated potassium channel 4
• NCBI Official Symbol: Hcn4
• NCBI Protein Information: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4; hyperpolarization-activated, cyclic nucleotide-gated K+ 4
• UniProt Protein Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• Protein Family: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel
• UniProt Gene Name: Hcn4
• UniProt Entry Name: HCN4_RAT

NCBI Description

may act as a neuronal pacemaker channel; may play a role in neurotransmitter release [RGD, Feb 2006]

Uniprot Description

HCN4: Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli. Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2); also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors, in which case it is considered to be a congenital disorder. Defects in HCN4 are the cause of Brugada syndrome type 8 (BRGDA8). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the potassium channel HCN family.

Protein type: Channel, cation; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: neuron projection; cell soma; perinuclear region of cytoplasm; basolateral plasma membrane; integral to plasma membrane; plasma membrane; terminal button; intrinsic to plasma membrane

Molecular Function: identical protein binding; voltage-gated potassium channel activity; protein binding; intracellular cAMP activated cation channel activity; potassium channel activity; voltage-gated sodium channel activity; cAMP binding

Biological Process: regulation of synaptic transmission; regulation of membrane potential; in utero embryonic development; regulation of heart rate; regulation of heart contraction; cation transport