Mouse KCNQ4 Monoclonal Antibody | anti-KCNQ4 antibody

KCNQ4 Antibody: ATTO 594

Cat. Num. AAA800338

• Gene Names: KCNQ4; DFNA2; KV7.4; DFNA2A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence
• Synonyms: KCNQ4; Monoclonal Antibody; KCNQ4 Antibody: ATTO 594; DFNA2; KQT like 4; Kv7.4; voltage gated potassium channel subunit Kv7.4; voltage gated potassium channel antibodies; anti-KCNQ4 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S43-6
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 695

• Applicable Applications for anti-KCNQ4 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: ATTO 594
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Western Blot (WB)

(Western Blot analysis of Rat tissue lysate showing detection of KCNQ4 protein using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

Related Product Information for anti-KCNQ4 antibody

Background Info: Detects ~77kDa.

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, the protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability (3), particularly in sensory cells of the cochlea (4). The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug (5).

Product Categories/Family for anti-KCNQ4 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Hernandez C.C., Zaiko O., Tolstykh G.P., Shapiro M.S. (2008) J Physiol. 586(7): 1811-1821. 4. Kharkovets T., et al. (2006) EMBO J. 25(3): 642-652. 5. Tatulian L., Delmas P., Abogadie F.C., Brown D.A. (2001) J Neuroscience. 21(15): 5535-5545.

NCBI and Uniprot Product Information

• NCBI GI #: 26638653
• NCBI GeneID: 9132
• NCBI Accession #: NP_004691.2
• NCBI GenBank Nucleotide #: NM_004700.3
• UniProt Accession #: P56696; O96025
• Molecular Weight: 71,196 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily KQT member 4 isoform a
• NCBI Official Synonym Full Names: potassium voltage-gated channel, KQT-like subfamily, member 4
• NCBI Official Symbol: KCNQ4
• NCBI Official Synonym Symbols: DFNA2; KV7.4; DFNA2A
• NCBI Protein Information: potassium voltage-gated channel subfamily KQT member 4; potassium channel KQT-like 4; potassium channel subunit alpha KvLQT4
• UniProt Protein Name: Potassium voltage-gated channel subfamily KQT member 4
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNQ4
• UniProt Entry Name: KCNQ4_HUMAN

NCBI Description

The protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. The encoded protein can form a homomultimeric potassium channel or possibly a heteromultimeric channel in association with the protein encoded by the KCNQ3 gene. Defects in this gene are a cause of nonsyndromic sensorineural deafness type 2 (DFNA2), an autosomal dominant form of progressive hearing loss. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KCNQ4: Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinnic receptors. Defects in KCNQ4 are the cause of deafness autosomal dominant type 2A (DFNA2A). DFNA2A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: voltage-gated potassium channel complex; integral to membrane; plasma membrane; basal plasma membrane

Molecular Function: potassium channel activity; delayed rectifier potassium channel activity

Biological Process: synaptic transmission; inner ear morphogenesis; sensory perception of sound; potassium ion transport

Disease: Deafness, Autosomal Dominant 2a