Mouse anti-Human HCG-beta Monoclonal Antibody | anti-CGB antibody

HCG-beta (Pregnancy & Choriocarcinoma Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438435

• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: HCG-beta; Monoclonal Antibody; HCG-beta (Pregnancy & Choriocarcinoma Marker) Mouse Monoclonal Antibody; CG-beta; CGB3; CGB5; CGB7; CGB8; Choriogonadotropin Subunit beta; hCGB; anti-CGB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SPM529
• Specificity: This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-CGB antibody: Immunohistochemistry (IHC) Formalin
• Application Notes: Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml; 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic, Secreted
• Immunogen: Recombinant hCG beta protein
• Hu-Chromosome Location: 19q13.33
• Positive Control: Placenta
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Placenta stained with hCG beta Monoclonal Antibody (SPM529).)

References

Cocquebert M et. al. Am J Physiol Endocrinol Metab. 2012;303(8):E950-8

NCBI and Uniprot Product Information

• NCBI GI #: 116184
• NCBI GeneID: 94115
• UniProt Accession #: P01233
• Molecular Weight: 22kDa
• NCBI Official Synonym Full Names: chorionic gonadotropin beta subunit 8
• NCBI Official Symbol: CGB8
• NCBI Protein Information: chorionic gonadotropin, beta polypeptide 8
• Protein Family: Gonadotropin

NCBI Description

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 8 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CGB: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Belongs to the glycoprotein hormones subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.32