Mouse anti-Human GROS1 Monoclonal Antibody | anti-GROS1 antibody

GROS1 (Growth Suppressor 1, Leucine- and Proline-enriched Proteoglycan 1, LEPRE1, Leprecan-1, MGC117314, OI8, Prolyl 3-hydroxylase 1, P3H1, PSEC0109) (PE)

Cat. Num. AAA6158085

• Gene Names: P3H1; OI8; GROS1; LEPRE1
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GROS1; Monoclonal Antibody; GROS1 (Growth Suppressor 1; Leucine- and Proline-enriched Proteoglycan 1; LEPRE1; Leprecan-1; MGC117314; OI8; Prolyl 3-hydroxylase 1; P3H1; PSEC0109) (PE); anti-GROS1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3C7
• Specificity: Recognizes human LEPRE1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1544

• Applicable Applications for anti-GROS1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1-100 from human LEPRE1 (AAH15309) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: DPRVREVMNQNLAYYAAMLGEEHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIE
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37kD).)

Western Blot (WB)

(Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody. Lane 1: LEPRE1 transfected lysate (46kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)

Western Blot (WB)

(LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)

Product Categories/Family for anti-GROS1 antibody

Antibodies; Extracellular Matrix Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1624806648
• NCBI GeneID: 64175
• NCBI Official Full Name: Homo sapiens leucine proline-enriched proteoglycan (leprecan) 1, mRNA
• NCBI Official Synonym Full Names: prolyl 3-hydroxylase 1
• NCBI Official Symbol: P3H1
• NCBI Official Synonym Symbols: OI8; GROS1; LEPRE1
• NCBI Protein Information: prolyl 3-hydroxylase 1
• Protein Family: 10 kDa chaperonin

NCBI Description

This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq, Aug 2011]