Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Mouse anti-Human GROS1 Monoclonal Antibody | anti-Lepre1 antibody
GROS1 (Growth Suppressor 1, Leucine- and Proline-enriched Proteoglycan 1, LEPRE1, Leprecan-1, MGC117314, OI8, Prolyl 3-hydroxylase 1, P3H1, PSEC0109)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 6ug/ml
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Western Blot (WB)
(Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody.|Lane 1: LEPRE1 transfected lysate (46kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody.|Lane 1: LEPRE1 transfected lysate (46kD).|Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data
(Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)
Western Blot (WB)
(LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)
Western Blot (WB)
(LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)
NCBI and Uniprot Product Information
Uniprot Description
LEPRE1: Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro- Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts. Defects in LEPRE1 are the cause of osteogenesis imperfecta type 8 (OI8). A connective tissue disorder characterized by disproportionate short stature, severe osteoporosis, shortening of the long bones, white sclerae, a round face and a short barrel-shaped chest. Belongs to the leprecan family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.7; Oxidoreductase; Cell development/differentiation; Secreted; Secreted, signal peptide
Cellular Component: proteinaceous extracellular matrix; membrane; endoplasmic reticulum; cytoplasm; extracellular region; plasma membrane; basement membrane; nucleus
Molecular Function: procollagen-proline 3-dioxygenase activity; dioxygenase activity; L-ascorbic acid binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity
Biological Process: regulation of protein secretion; collagen fibril organization; protein folding; protein stabilization; collagen metabolic process; peptidyl-proline hydroxylation; protein amino acid hydroxylation; regulation of ossification; cell growth
Research Articles on Lepre1
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Product Notes
The Lepre1 lepre1 (Catalog #AAA6008049) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GROS1 (Growth Suppressor 1, Leucine- and Proline-enriched Proteoglycan 1, LEPRE1, Leprecan-1, MGC117314, OI8, Prolyl 3-hydroxylase 1, P3H1, PSEC0109) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GROS1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 6ug/ml. Researchers should empirically determine the suitability of the Lepre1 lepre1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DPRVREVMNQ NLAYYAAMLG EEHTRSIGPR ESAKEYRQRS LLEKELLFFA YDVFGIPFVD PDSWTPEEVI PKRLQEKQKS ERETAVRISQ EIGNLMKEIE. It is sometimes possible for the material contained within the vial of "GROS1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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